Melief C J, Schweitzer M, Zeylemaker W P, Verhagen E H, Eijsvoogel V P
Clin Exp Immunol. 1973 Sep;15(1):131-43.
The responses of lymphoid cells from the blood of children with acute lymphatic leukaemia to stimulation with phytohaemagglutinin, pokeweed mitogen, allogeneic lymphocytes and a cocktail of five different antigens were studied. In addition the stimulatory capacity in mixed lymphocyte culture was investigated as well as the formation of rosettes with sheep erythrocytes which were either uncoated or coated with antibody and complement. Whereas lymphocytes from acute lymphatic leukaemia patients in remission responded well to all stimuli tested, lymphoid cells from all seven patients in leukaemic stages with at least 80% lymphatic cells in the differential leucocyte count uniformly displayed strongly reduced or even absent responses. The phytohaemagglutinin response of cells from leukaemic stages was not only reduced but also delayed and the stimulatory capacity in mixed lymphocyte culture was always stronger than the responding capacity. These data indicate that the responses of cells from leukaemic stages are due to the presence of a small proportion of residual normal lymphocytes whereas the leukaemic cells themselves are unable to respond. The stimulatory capacity in mixed lymphocyte culture was reduced in four cases. The cells from two patients, however, displayed increased stimulatory capacity. The cells from different patients varied strongly with respect to the presence of surface markers as tested by rosette formation with sheep erythrocytes either uncoated or coated with antibody and complement.
研究了急性淋巴细胞白血病患儿血液中淋巴细胞对植物血凝素、商陆有丝分裂原、同种异体淋巴细胞以及五种不同抗原混合物刺激的反应。此外，还研究了混合淋巴细胞培养中的刺激能力以及与未包被或包被有抗体和补体的绵羊红细胞形成玫瑰花结的情况。处于缓解期的急性淋巴细胞白血病患者的淋巴细胞对所有测试刺激反应良好，而在白血病期的所有七名患者中，白细胞分类计数中至少80%为淋巴细胞的淋巴样细胞均一致显示反应强烈降低甚至缺失。白血病期细胞对植物血凝素的反应不仅降低，而且延迟，混合淋巴细胞培养中的刺激能力总是强于反应能力。这些数据表明，白血病期细胞的反应是由于存在一小部分残留的正常淋巴细胞，而白血病细胞本身无反应能力。在四例中，混合淋巴细胞培养中的刺激能力降低。然而，两名患者的细胞显示出增强的刺激能力。通过与未包被或包被有抗体和补体的绵羊红细胞形成玫瑰花结测试，不同患者的细胞在表面标志物的存在方面差异很大。
