体外淋巴细胞转化。3. 混合淋巴细胞培养中的刺激机制。
Lymphocyte transformation in vitro. 3. Mechanism of stimulation in the mixed lymphocyte culture.
作者信息
Schellekens P T, Eijsvoogel V P
出版信息
Clin Exp Immunol. 1970 Aug;7(2):229-39.
Abstract
In view of the correlation between the mixed lymphocyte culture and the HL-A locus, experiments were performed to determine whether the reactivity in the mixed lymphocyte culture is elicited by the HL-A antigens as such. Fresh lymphocytes were mixed with either allogeneic fibroblasts or allogeneic lymphocytes treated with various metabolic inhibitors or by heating which abolishes their capacity to tranfsorm . No stimulation of the normal untreated lymphocytes was observed in any of these mixed cultures. However, the HL-A antigens 4b and 7b were not affected quantitatively by this treatment as determined serologically. The conclusion is drawn that mixed lymphocyte culture reactivity is not merely a reaction to HL-A antigens, and the consequences of this are discussed.
摘要
鉴于混合淋巴细胞培养与HL - A位点之间的相关性，进行了实验以确定混合淋巴细胞培养中的反应性是否由HL - A抗原本身引发。将新鲜淋巴细胞与同种异体成纤维细胞或用各种代谢抑制剂处理或加热处理（从而消除其转化能力）的同种异体淋巴细胞混合。在任何这些混合培养物中均未观察到对正常未处理淋巴细胞的刺激。然而，通过血清学测定，HL - A抗原4b和7b在数量上不受此处理的影响。得出的结论是，混合淋巴细胞培养反应性不仅仅是对HL - A抗原的反应，并讨论了其后果。
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