不使DNA结合活性失活的乳糖阻遏物纯化。
Lac repressor purification without inactivation of DNA binding activity.
作者信息
Rosenberg J M, Khallai O B, Kopka M L, Dickerson R E, Riggs A D
出版信息
Nucleic Acids Res. 1977 Mar;4(3):567-72. doi: 10.1093/nar/4.3.567.
Abstract
A procedure has been developed which eliminates the commonly observed inactivation of the DNA binding activity of the lac repressor during purification. The operator binding activity of the repressor obtained by this method is 100 +/- 10%. The repressor can be stored frozen indefinitely without losing its affinity for DNA.
摘要
已开发出一种方法，可消除纯化过程中常见的乳糖阻遏物DNA结合活性的失活现象。通过这种方法获得的阻遏物的操纵子结合活性为100±10%。该阻遏物可以无限期冷冻保存而不会丧失其对DNA的亲和力。
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On the assay, isolation and characterization of the lac repressor.关于乳糖阻遏物的测定、分离及特性分析。
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Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.乳糖操纵子类似物：乳糖操纵子中的溴脱氧尿苷取代影响乳糖阻遏物的解离速率。
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Equilibrium and kinetic studies of Escherichia coli lac repressor-inducer interactions.大肠杆菌乳糖阻遏物与诱导剂相互作用的平衡和动力学研究。
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