动力学校对：一种减少生物合成过程中错误以实现高特异性的新机制。
Kinetic proofreading: a new mechanism for reducing errors in biosynthetic processes requiring high specificity.
作者信息
Hopfield J J
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4135-9. doi: 10.1073/pnas.71.10.4135.
Abstract
The specificity with which the genetic code is read in protein synthesis, and with which other highly specific biosynthetic reactions take place, can be increased above the level available from free energy differences in intermediates or kinetic barriers by a process defined here as kinetic proofreading. A simple kinetic pathway is described which results in this proofreading when the reaction is strongly but nonspecifically driven, e.g., by phosphate hydrolysis. Protein synthesis, amino acid recognition, and DNA replication, all exhibit the features of this model. In each case, known reactions which otherwise appear to be useless or deleterious complications are seen to be essential to the proofreading function.
摘要
在蛋白质合成中读取遗传密码以及发生其他高度特异性生物合成反应时的特异性，可通过此处定义为动力学校对的过程，提高到高于中间体自由能差异或动力学障碍所能提供的水平。描述了一条简单的动力学途径，当反应由磷酸水解等强烈但非特异性驱动时，该途径会导致这种校对。蛋白质合成、氨基酸识别和DNA复制均表现出该模型的特征。在每种情况下，那些否则看似无用或有害的复杂反应，实际上对于校对功能至关重要。
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