钙离子载体A23187的促有丝分裂特性。
Mitogenic properties of a calcium ionophore, A23187.
作者信息
Luckasen J R, White J G, Kersey J H
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):5088-90. doi: 10.1073/pnas.71.12.5088.
Abstract
A23187 is a carboxylic antibiotic that selectively transfers calcium, magnesium, and other divalent cations across biologic membranes. This ionophore was found to produce morphologic blast transformation, DNA synthesis, and mitosis in human lymphocytes. Several hours of ionophore-cell contact were necessary to produce optimal mitogenesis. The effects were highly dependent on the presence of extracellular calcium and much less dependent on extracellular magnesium. Lanthanum chloride prevented the development of the observed ionophore effects. Results are consistent with the hypothesis that under physiologic conditions the interaction of antigens or mitogens with specific receptors at the lymphocyte membrane initiates events that alter calcium fluxes and result in increased cytoplasmic calcium. Increased cytoplasmic calcium is postulated to play a central role in the generation of surface-to-nuclear signals that initiate the process of DNA synthesis and cell division.
摘要
A23187是一种羧酸抗生素，可选择性地将钙、镁和其他二价阳离子转运穿过生物膜。已发现这种离子载体可使人类淋巴细胞发生形态学的原始细胞转化、DNA合成和有丝分裂。离子载体与细胞接触数小时对于产生最佳的有丝分裂原作用是必要的。这些效应高度依赖于细胞外钙的存在，而对细胞外镁的依赖性则小得多。氯化镧可阻止所观察到的离子载体效应的产生。结果与以下假设一致：在生理条件下，抗原或促细胞分裂剂与淋巴细胞膜上特定受体的相互作用引发改变钙通量的事件，并导致细胞质钙增加。据推测，细胞质钙增加在启动DNA合成和细胞分裂过程的表面到核信号的产生中起核心作用。
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