黄曲霉毒素与土曲霉毒素的鉴别
Differentiation of aflatoxins from territrems.
作者信息
Ling K H, Yang C K, Huang H C
出版信息
Appl Environ Microbiol. 1979 Mar;37(3):358-61. doi: 10.1128/aem.37.3.358-361.1979.
Three methods were adopted for differentiation of aflatoxins B1 and B2 from territrems A and B. They were as follows. (i) Then-layer chromatography coupled with chemical confirmation. A significant decrease in the Rf value of trifluoroacetic acid-treated aflatoxin B1 developed in chloroform-acetone (85:15, vol/vol) was satisfactory in differentiating this toxin from the other three. (ii) High-pressure liquid chromatography monitored synchronously at two wavelengths, 365 and 335 nm. The ratio derived from this double-wavelengh detection could serve as an indicator of the presence of each toxin. (iii) Velasco's flurotoxin meter method, which is used for the determination of aflatoxins within the range of 0 to 50 ng/ml, was not significantly affected by territrems even when they were present in quantities at the microgram-per-milliliter level.
采用了三种方法来区分黄曲霉毒素B1和B2与土曲霉毒素A和B。具体如下：(i) 薄层色谱法结合化学确证。在氯仿 - 丙酮（85:15，体积/体积）中展开的经三氟乙酸处理的黄曲霉毒素B1的比移值显著降低，这对于将该毒素与其他三种毒素区分开来是令人满意的。(ii) 在365和335 nm两个波长下同步监测的高压液相色谱法。这种双波长检测得出的比率可作为每种毒素存在的指标。(iii) 贝拉斯科荧光毒素测定仪法，用于测定0至50 ng/ml范围内的黄曲霉毒素，即使土曲霉毒素以微克/毫升水平存在时，该方法也不会受到显著影响。
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