用于改进土曲霉毒素A和B分离与提纯的溶剂系统。
Solvent systems for improved isolation and separation of territrems A and B.
作者信息
Ling K H, Yang C K, Kuo C A, Kuo M D
出版信息
Appl Environ Microbiol. 1982 Oct;44(4):860-3. doi: 10.1128/aem.44.4.860-863.1982.
Abstract
Territrems A and B, tremorgenic mycotoxins in the rice culture of Aspergillus terreus, were extracted with hot chloroform. The toxins were cleaned on a silica gel column by direct adsorption-concentration of the extracts followed by desorption with chloroform-acetone (9:1, vol/vol). Crude toxin mixtures were separated by silica gel column chromatography and eluted with benzene-ethyl acetate (65:35, vol/vol). The method gave 112 mg of territrem A, 379 mg of territrem B, and 80 mg of their mixture from 4 kg of rice per batch. The criteria of extraction, cleanup, and separation are provided.
摘要
土曲霉大米培养物中的震颤毒素A和B（两种震颤性霉菌毒素）用热氯仿进行提取。毒素在硅胶柱上进行净化，方法是直接吸附浓缩提取物，随后用氯仿 - 丙酮（9:1，体积/体积）进行解吸。粗毒素混合物通过硅胶柱色谱法分离，并用苯 - 乙酸乙酯（65:35，体积/体积）洗脱。该方法每批从4千克大米中得到112毫克震颤毒素A、379毫克震颤毒素B及其80毫克混合物。文中提供了提取、净化和分离的标准。
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