一种具有B细胞促有丝分裂活性的合成糖脂。
A synthetic glycolipid with B-cell mitogenic activity.
作者信息
Rosenstreich D L, Asselineau J, Mergenhagen S E, Nowotny A
出版信息
J Exp Med. 1974 Nov 1;140(5):1404-9. doi: 10.1084/jem.140.5.1404.
A synthetic glycolipid, N-palmitoyl-D-glucosamine, (NPG) was found to be a potent B-cell mitogen, that stimulated spleen cells from nu/nu as well as from normal mice. The proper dispersion of the water insoluble preparation is critical for the elicitation of this mitogenic effect. Limulus lysate clotting assay indicated that the NPG preparation either contains only 0.001% endotoxin contamination, or that NPG itself is 10(-5) times less active in this assay than purified endotoxic LPS. Since such low levels of endotoxin concentration are not mitogenic, it is concluded that synthetic N-palmitoyl-D-glucosamine, when properly dispersed, is itself a B-cell mitogen.
一种合成糖脂，N-棕榈酰-D-葡萄糖胺（NPG）被发现是一种有效的B细胞有丝分裂原，它能刺激无胸腺裸鼠以及正常小鼠的脾细胞。水不溶性制剂的适当分散对于引发这种有丝分裂效应至关重要。鲎试剂凝血试验表明，NPG制剂要么仅含有0.001%的内毒素污染，要么NPG本身在该试验中的活性比纯化的内毒素脂多糖低10^(-5)倍。由于如此低水平的内毒素浓度没有促有丝分裂作用，因此得出结论，当适当分散时，合成的N-棕榈酰-D-葡萄糖胺本身就是一种B细胞有丝分裂原。
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