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青蛙肌肉中质子核磁共振弛豫测量

Proton nuclear magnetic resonance relaxation measurements in frog muscle.

作者信息

Finch E D, Homer L D

出版信息

Biophys J. 1974 Dec;14(12):907-21. doi: 10.1016/S0006-3495(74)85958-8.

Abstract

Proton nuclear magnetic resonance (NMR) relaxation measurements are reported for frog muscle as a function of temperature and Larmor frequency. Each T(1rho), T(2), and T(1) measurement covered a time domain sufficient to identify the average relaxation time for most intracellular water. Using regression analysis the data were fit with a model where intracellular water molecules are exchanging between a large compartment in which mobility is similar to ordinary water and a small compartment in which motion is restricted. The regression results suggest that: the restricted compartment exhibits a distribution of motions skewed toward that of free water; the residence time of water molecules in the restricted compartment is approximately 1 ms; and, the activation entropy for some water molecules in the restricted compartment is negative.

摘要

报告了青蛙肌肉的质子核磁共振(NMR)弛豫测量结果,该结果是温度和拉莫尔频率的函数。每次T(1ρ)、T(2)和T(1)测量都覆盖了一个足以确定大多数细胞内水的平均弛豫时间的时域。使用回归分析,数据与一个模型拟合,在该模型中,细胞内水分子在一个流动性与普通水相似的大隔室和一个运动受限的小隔室之间交换。回归结果表明:受限隔室表现出偏向自由水运动的运动分布;水分子在受限隔室中的停留时间约为1毫秒;并且,受限隔室中一些水分子的活化熵为负。

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引用本文的文献

本文引用的文献

1
NUCLEAR MAGNETIC RESONANCE STUDIES OF LIVING MUSCLE.活体肌肉的核磁共振研究
Science. 1965 Feb 12;147(3659):738-9. doi: 10.1126/science.147.3659.738.
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Proton magnetic resonance studies of water in slime mould plasmodia.
Aust J Biol Sci. 1971 Jun;24(3):497-507. doi: 10.1071/bi9710497.

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