通过与胸腺提取物进行体外孵育，将人骨髓细胞分化为T淋巴细胞。
Differentiation of human bone marrow cells into T lymphocytes by in vitro incubation with thymic extracts.
作者信息
Touraine J L, Incefy G S, Touraine F, Rho Y M, Good R A
出版信息
Clin Exp Immunol. 1974 May;17(1):151-8.
Abstract
Extracts of human or calf thymus influence differentiation of human bone marrow cells . Incubation of a putative stem cell fraction of human marrow with extracts of thymus for a period of 2 hr led to the appearance of lymphocytes with surface antigens recognized by a highly specific anti-T-cell antiserum. To a lesser degree, development of lymphocytes having the capacity to form rosettes with sheep erythrocytes was observed. Treatment of the so-called stem cell fraction with thymic extracts did not yield cells responsive to mitogenic influences of phyto-haemagglutinin or allogeneic cells. This model should permit further analyses of immunodeficiency diseases and provide a useful technique for purification and analysis of thymus extracts active with human haematopoietic cells.
摘要
人或小牛胸腺提取物会影响人骨髓细胞的分化。将人骨髓的假定干细胞部分与胸腺提取物孵育2小时，会导致出现带有被高度特异性抗T细胞抗血清识别的表面抗原的淋巴细胞。在较小程度上，观察到了具有与绵羊红细胞形成玫瑰花结能力的淋巴细胞的发育。用胸腺提取物处理所谓的干细胞部分，并未产生对植物血凝素或同种异体细胞的促有丝分裂影响有反应的细胞。该模型应有助于对免疫缺陷疾病进行进一步分析，并为纯化和分析对人造血细胞有活性的胸腺提取物提供一种有用的技术。
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