Vogel J E, Incefy G S, Good R A
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1175-8. doi: 10.1073/pnas.72.3.1175.
A small population of human marrow cells has been shown to be differentiated in vitro by thymic extract into cells bearing T-lymphocyte (thymus-derived lymphocyte) characteristics. By a similar method, the differentiation of human peripheral blood lymphocytes has been studied. A discontinuous gradient of bovine serum albumin was used to isolate lymphocytes into four layers and cells from layers I and III demonstrated the greatest potential for differentiation by human thymic extract. Appearance of T-lymphocyte characteristics was recognized by the spontaneous E-rosette technique with sheep erythrocytes. Ability of human marrow cells to be differentiated under the influence of human thymic extract was abolished by specific inhibitors of nucleic acid synthesis, however, had no inhibitory effect on the maturation of peripheral blood lymphocytes during a 2 hr incubation with human thymic extract but puromycin, an inhibitor of protein synthesis, abolished this differentiative step in cells of layer I. It is suggested from these studies that many of the cells in peripheral blood that are differentiable by thymic extract are at a stage of maturation more advanced than those in human marrow that are also differentiable by thymic extract.
一小部分人类骨髓细胞已被证明在体外可通过胸腺提取物分化为具有T淋巴细胞（胸腺来源淋巴细胞）特征的细胞。通过类似的方法，对人类外周血淋巴细胞的分化进行了研究。使用不连续的牛血清白蛋白梯度将淋巴细胞分离成四层，来自第一层和第三层的细胞在人类胸腺提取物作用下表现出最大的分化潜力。通过与绵羊红细胞的自发E花环技术识别T淋巴细胞特征的出现。核酸合成的特异性抑制剂消除了人类骨髓细胞在人类胸腺提取物影响下的分化能力，然而，在与人类胸腺提取物孵育2小时期间，其对外周血淋巴细胞的成熟没有抑制作用，但蛋白质合成抑制剂嘌呤霉素消除了第一层细胞中的这一分化步骤。从这些研究中可以看出，外周血中许多可被胸腺提取物分化的细胞比人类骨髓中同样可被胸腺提取物分化的细胞处于更高级的成熟阶段。
