DNA合成的起始：φX174复制型的合成需要对利福平耐药的RNA合成。
Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin.
作者信息
Schekman R, Wickner W, Westergaard O, Brutlag D, Geider K, Bertsch L L, Kornberg A
出版信息
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691-5. doi: 10.1073/pnas.69.9.2691.
Abstract
Conversion of single-stranded DNA of phage varphiX174 to the double-stranded replicative form in Escherichia coli uses enzymes essential for initiation and replication of the host chromosome. These enzymes can now be purified by the assay that this phage system provides. The varphiX174 conversion is distinct from that of M13. The reaction requires different host enzymes and is resistant to rifampicin and streptolydigin, inhibitors of RNA polymerase. However, RNA synthesis is essential for varphiX174 DNA synthesis: the reaction is inhibited by low concentrations of actinomycin D, all four ribonucleoside triphosphates are required, and an average of one phosphodiester bond links DNA to RNA in the isolated double-stranded circles. Thus, we presume that, as in the case of M13, synthesis of a short RNA chain primes the synthesis of a replicative form by DNA polymerase. Initiation of DNA synthesis by RNA priming is a mechanism of wide significance.
摘要
噬菌体φX174的单链DNA在大肠杆菌中转化为双链复制形式，需要宿主染色体起始和复制所必需的酶。现在可以通过该噬菌体系统提供的检测方法来纯化这些酶。φX174的转化与M13的不同。该反应需要不同的宿主酶，并且对利福平及链霉溶菌素（RNA聚合酶抑制剂）具有抗性。然而，RNA合成对于φX174 DNA合成至关重要：该反应被低浓度的放线菌素D抑制，所有四种核糖核苷三磷酸都是必需的，并且在分离的双链环中平均有一个磷酸二酯键将DNA与RNA相连。因此，我们推测，与M13的情况一样，短RNA链的合成通过DNA聚合酶引发复制形式的合成。通过RNA引发启动DNA合成是一种具有广泛意义的机制。
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