Solomon J B, Riddell G S, Whyte D F
Immunology. 1972 Feb;22(2):219-26.
Young rats do not respond to antigenic stimulation with sheep erythrocytes for the first 2 weeks of post-natal life. However, if mouse erythrocytes were used as antigen a plaque-forming cell (PFC) response was sometimes detectable as early as 4 days after birth. In order to demonstrate that undetectable trace amounts of anti-sheep antibody of maternal origin were causing the suppressive delay in PFC response to sheep erythrocytes, means of artificially suppressing the anti-mouse PFC response were studied. Hyperimmunization of pregnant rats with mouse erythrocytes resulted in the transmission of sufficient anti-mouse antibody to the offspring to bring about complete and specific suppression of their PFC response for up to 8 weeks after birth. High dilutions of IgM or IgG fractions of anti-mouse rat sera administered to 6-day-old rats 24 hours before they were injected with mouse erythrocyte antigen caused complete suppression of the relatively weak PFC response. This model can be used to detect trace amounts of antibody and is about 400 times more sensitive than conventional haemagglutination techniques.
幼鼠在出生后的前两周内对绵羊红细胞的抗原刺激无反应。然而，如果使用小鼠红细胞作为抗原，早在出生后4天有时就能检测到空斑形成细胞（PFC）反应。为了证明母源的不可检测的微量抗绵羊抗体导致了对绵羊红细胞的PFC反应的抑制延迟，研究了人工抑制抗小鼠PFC反应的方法。用小鼠红细胞对怀孕大鼠进行超免疫，会导致足够的抗小鼠抗体传递给后代，从而在出生后长达8周的时间内完全特异性地抑制它们的PFC反应。在6日龄大鼠注射小鼠红细胞抗原前24小时，给它们施用高稀释度的抗小鼠大鼠血清的IgM或IgG组分，会完全抑制相对较弱的PFC反应。该模型可用于检测微量抗体，其灵敏度比传统血凝技术高约400倍。
