Woodworth-Gutai M, Israel V, Levine M
J Virol. 1972 May;9(5):746-51. doi: 10.1128/JVI.9.5.746-751.1972.
Extracts from P22-infected and uninfected cultures of Salmonella typhimurium were subjected to deoxyribonucleic acid (DNA)-cellulose and diethylaminoethyl-cellulose chromatography. Comparison of the elution patterns revealed that in infected cells there is a decrease in the amount of nuclease activity specific for denatured DNA and an increase in the amount of nuclease activity specific for native DNA. The latter activity was shown to differ from a similar host enzyme in Mg(2+), Mn(2+), and pH optima. This new activity is not found after infection of a lysogen with a nonvirulent phage or after infection under nonpermissive conditions with P22ts25.1 (a mutant in gene 25 that carries out no known functions other than adsorption and injection) and thus appears to be specified by the phage genome.
对鼠伤寒沙门氏菌受P22感染和未感染培养物的提取物进行脱氧核糖核酸（DNA）-纤维素和二乙氨基乙基纤维素色谱分析。洗脱模式的比较显示，在受感染细胞中，对变性DNA具有特异性的核酸酶活性量减少，而对天然DNA具有特异性的核酸酶活性量增加。后者的活性在镁离子（Mg²⁺）、锰离子（Mn²⁺）和最适pH值方面与一种类似的宿主酶不同。在用无毒噬菌体感染溶原菌后，或在用P22ts25.1（基因25中的一个突变体，除吸附和注入外无其他已知功能）在非允许条件下感染后，未发现这种新活性，因此它似乎是由噬菌体基因组所决定的。
