鼠伤寒沙门氏菌一种主要脱氧核糖核酸聚合酶活性缺陷型突变体的分离与鉴定
Isolation and characterization of a mutant of Salmonella typhimurium deficient in a major deoxyribonucleic acid polymerase activity.
作者信息
Whitfield H J, Levine G
出版信息
J Bacteriol. 1973 Oct;116(1):54-8. doi: 10.1128/jb.116.1.54-58.1973.
Abstract
A mutant of Salmonella typhimurium strain LT2 that is deficient in a major deoxyribonucleic acid (DNA) polymerase activity has been isolated and characterized. This mutant resembles the pol mutants of E. coli in that it has low DNA polymerase activity and it is sensitive to methyl methane sulfonate as well as ultraviolet irradiation. Revertants selected for methyl methane sulfonate resistance are no longer sensitive to ultraviolet irradiation and contain normal DNA polymerase levels. No direct role in replication can be ascribed to this polymerase activity since cells grow well in its absence. In addition, the LT2 plasmid has been shown to exist in the mutant strain.
摘要
一株鼠伤寒沙门氏菌LT2的突变体已被分离和鉴定，该突变体缺乏一种主要的脱氧核糖核酸（DNA）聚合酶活性。此突变体与大肠杆菌的pol突变体相似，其DNA聚合酶活性较低，对甲基磺酸甲酯以及紫外线照射敏感。筛选出的对甲基磺酸甲酯具有抗性的回复突变体不再对紫外线照射敏感，且DNA聚合酶水平正常。由于细胞在缺乏这种聚合酶活性的情况下仍能良好生长，因此不能将此聚合酶活性直接归因于复制过程。此外，已证明LT2质粒存在于突变菌株中。
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