Craig W A, Kunin C M
Appl Microbiol. 1972 May;23(5):919-22. doi: 10.1128/am.23.5.919-922.1972.
A new dip-inoculum method for quantitative urine culture is described which utilizes a dual-chambered plastic "paddle" housing both a general purpose and differential medium. Comparative bacterial counts of 1,000 clinical specimens using the pour plate and this device were identical in 82.9% and within a factor of five in 95.6%. The "paddle" detected all but 19 of 258 specimens (92.6%) with 100,000 or greater colonies per ml. This simple, convenient method should allow more extensive use of quantitative urine culture in the diagnosis and follow-up of patients with urinary tract infections in office practice. It should not be considered as a substitute for the more definitive pour plate method or for standard methods for characterization of bacteriological species when more exact information is required.
本文描述了一种新的定量尿培养浸种法,该方法使用一种双腔塑料“平板”,其中包含通用培养基和鉴别培养基。使用倾注平板法和该装置对1000份临床标本进行的细菌计数比较中,82.9%的结果相同,95.6%的结果相差不超过五倍。该“平板”检测出每毫升菌落数为100,000或更多的258份标本中,除19份外的所有标本(92.6%)。这种简单、方便的方法应能在门诊实践中更广泛地用于尿路感染患者的诊断和随访中的定量尿培养。当需要更确切的信息时,不应将其视为更确切的倾注平板法或细菌学菌种鉴定标准方法的替代方法。