Moffat C M, Britt M R, Burke J P
Appl Microbiol. 1974 Jul;28(1):95-9. doi: 10.1128/am.28.1.95-99.1974.
A new dip-inoculum method for detecting bacteriuria which utilizes dehydrated media pads and a nitrite pad attached to a small plastic strip was evaluated in hospitalized patients. Discrepant interpretations were made by independent observers in 9.3% of the specimens with > 10(5) colonies per ml. The media pads failed to support growth of yeast and gave variable results with Staphylococcus epidermidis and non-group D streptococci. False-negative culture results commonly occurred if the patients were receiving antibiotics. The nitrite test occasionally remained positive for brief periods after the elimination of bacteriuria by antibiotics. Conditions and drugs (especially phenazopyridine) which discolor urine interfered with reading both the culture and nitrite tests. Although not suitable for hospital use, or for monitoring therapy, the test strip is probably as reliable as the calibrated loop-streak plate culture for office screening.
一种利用脱水培养基垫和附着在小塑料条上的亚硝酸盐垫检测菌尿的新浸种接种法在住院患者中进行了评估。独立观察者对每毫升菌落数>10⁵的标本中有9.3%的标本解读存在差异。培养基垫不能支持酵母生长，对表皮葡萄球菌和非D群链球菌的检测结果不稳定。如果患者正在接受抗生素治疗，通常会出现假阴性培养结果。在通过抗生素消除菌尿后，亚硝酸盐试验偶尔会在短时间内保持阳性。使尿液变色的情况和药物（尤其是非那吡啶）会干扰培养和亚硝酸盐试验的读数。虽然该试纸条不适合医院使用或监测治疗，但对于门诊筛查来说，其可靠性可能与校准环划线平板培养法相当。
