McCoy R C
Am J Pathol. 1972 Sep;68(3):469-78.
Immunofluorescent evaluation of renal biopsies from 19 patients with lupus nephritis revealed nuclear localization of immunoglobulins (IgG and IgM) in 6 patients. Homogeneous, nuclear rim and speckled patterns of nuclear localization were observed. The extent of localization varied, with only occasional nuclei fluorescing in 1 case, whereas approximately 50% of the nuclei exhibited fluorescence in the most extreme case. The phenomenon of nuclear localization of immunoglobulins was not observed in immunofluorescent studies of 225 renal biopsies from patients with conditions other than lupus nephritis. The possibility that nuclear localization of immunoglobulins occurred artifactually in the 6 patients was considered and was discounted by determining antinuclear antibody titers on serum obtained concurrently with the renal biopsy Nuclear localization was not confined to areas of histologically evident parenchymal destruction, indicating that antinuclear antibodies do not react only with nuclear antigens after tissue breakdown, but may gain access to intracellular antigens prior to cell dissolution.
对19例狼疮性肾炎患者的肾活检组织进行免疫荧光评估发现，6例患者存在免疫球蛋白（IgG和IgM）的核定位。观察到核定位呈均匀、核周缘和斑点状模式。定位程度各不相同，1例仅偶尔有细胞核发出荧光，而在最严重的病例中约50%的细胞核呈现荧光。在对225例非狼疮性肾炎患者的肾活检组织进行免疫荧光研究时，未观察到免疫球蛋白核定位现象。考虑了这6例患者免疫球蛋白核定位可能是人为造成的，并通过测定与肾活检同时采集的血清中的抗核抗体滴度排除了这种可能性。核定位并不局限于组织学上明显的实质破坏区域，这表明抗核抗体并非仅在组织分解后才与核抗原发生反应，而是可能在细胞溶解之前就接触到细胞内抗原。
