Woods D R, Mosmann T R, Hanson S, Hendry D A
J Bacteriol. 1971 Nov;108(2):765-9. doi: 10.1128/jb.108.2.765-769.1971.
Stable, orange, acriflavine-resistant variants were selected by treatment of a wild-type, red, acriflavine-sensitive strain of Serratia marcescens with acriflavine. Visible, ultraviolet, infrared, and nuclear magnetic resonance spectra of purified pigment from the red strain were identical to those of the pigment from the orange strain, and the orange mutant was not due to a mutation affecting the structure of the pigment, prodigiosin. The color of the red strain was not affected by variations in pH between 5.0 and 8.0, whereas the color of the orange mutant changed from pink to orange over the same pH range. This variation was mimicked by the pH-induced variation in color of prodigiosin purified from either the red, wild-type or the orange, mutant strains. Density-gradient centrifugation of cell fragments after ultrasonic disintegration resulted in characteristic pigmented bands. Biochemical characterization of these pigmented bands showed that they contained pigment and a protein component, but no lipids, polysaccharides, sugars, glucosamine, or phosphates were detected. Further fractionation of these pigmented bands by zone electrophoresis on a sucrose density gradient indicated that some pigment in S. marcescens was specifically attached to protein components.
通过用吖啶黄处理粘质沙雷氏菌的野生型红色吖啶黄敏感菌株，筛选出稳定的橙色吖啶黄抗性变体。红色菌株纯化色素的可见光谱、紫外光谱、红外光谱和核磁共振光谱与橙色菌株色素的光谱相同，橙色突变体并非由于影响色素灵菌红素结构的突变所致。红色菌株的颜色在pH值5.0至8.0之间的变化不受影响，而橙色突变体的颜色在相同pH范围内从粉红色变为橙色。从红色野生型菌株或橙色突变体菌株纯化的灵菌红素的pH诱导颜色变化模拟了这种变化。超声破碎后细胞碎片的密度梯度离心产生了特征性的色素带。这些色素带的生化特性表明它们含有色素和一种蛋白质成分，但未检测到脂质、多糖、糖、氨基葡萄糖或磷酸盐。通过在蔗糖密度梯度上进行区带电泳对这些色素带进一步分级分离表明，粘质沙雷氏菌中的一些色素与蛋白质成分特异性结合。
