Noller H F, Chaires J B
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3115-8. doi: 10.1073/pnas.69.11.3115.
Kethoxal reacts with 30S ribosomal subunits to give totally inactive particles, as measured by in vitro protein synthesis. It is postulated that functional modification occurs at the binding site for transfer RNA since (a) loss of specific binding of transfer RNA, but not binding of messenger RNA, is simultaneous with loss of protein synthesis, and (b) loss of activity is inhibited by bound transfer RNA. By means of in vitro reconstitution and labeling techniques, it is found that loss of transfer RNA-binding activity is correlated with the modification of six or seven guanine residues in 16S RNA.
乙醛酸与30S核糖体亚基反应生成完全无活性的颗粒，这通过体外蛋白质合成来测定。据推测，功能修饰发生在转运RNA的结合位点，因为（a）转运RNA特异性结合的丧失，而非信使RNA的结合，与蛋白质合成的丧失同时发生，并且（b）结合的转运RNA可抑制活性的丧失。通过体外重组和标记技术发现，转运RNA结合活性的丧失与16S RNA中六个或七个鸟嘌呤残基的修饰相关。
