大肠杆菌中附加体整合的亚硝基胍测定法。
Nitrosoguanidine assay of episomal integration in Escherichia coli.
作者信息
Lloveres C P, Cerdá-Olmedo E
出版信息
J Bacteriol. 1973 Nov;116(2):527-30. doi: 10.1128/jb.116.2.527-530.1973.
Abstract
Mutations affecting utilization of lactose and resistance to the male-specific phages f1, f2, and Qbeta tend to occur simultaneously more often than expected by chance in Hfr strains whose origin of transfer is close to the genes for lactose utilization, but not in F(+) strains. Strains derived from the Hfr, but exhibiting poor ability to transfer early chromosomal genes, may or may not show this comutation phenomenon. These results support the concept that the F factor is integrated into the Hfr chromosome during vegetative growth, but is autonomous in the F(+) strains and could serve as an assay for episomal localization.
摘要
在转移起点靠近乳糖利用基因的高频重组（Hfr）菌株中，影响乳糖利用及对雄性特异性噬菌体f1、f2和Qβ抗性的突变往往比随机预期更频繁地同时发生，但在F(+)菌株中并非如此。源自Hfr但早期染色体基因转移能力较差的菌株，可能表现出也可能不表现出这种共突变现象。这些结果支持了这样一种概念，即F因子在营养生长期间整合到Hfr染色体中，但在F(+)菌株中是自主的，并且可以作为附加体定位的一种检测方法。
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