Israel V, Rosen H, Levine M
J Virol. 1972 Dec;10(6):1152-8. doi: 10.1128/JVI.10.6.1152-1158.1972.
Purified base-plate parts of bacteriophage P22 can bind to the host cell, Salmonella typhimurium. Although the reaction is reversible, a stable equilibrium is not formed between bound and unbound base-plate parts. This is because the binding sites on the cell, presumably the O antigens, are destroyed. The destruction of binding sites does not kill the cells, and, in fact, the binding sites are soon regenerated. The site-destroying activity reacts with P22 heads to make active phage and with antiserum made against purified phage. Therefore site-destroying activity is a characteristic of the base-plate parts and not some contaminant of the preparation.
噬菌体P22纯化的基板部分能与宿主细胞鼠伤寒沙门氏菌结合。虽然这种反应是可逆的,但在结合和未结合的基板部分之间不会形成稳定的平衡。这是因为细胞上的结合位点,推测是O抗原,被破坏了。结合位点的破坏不会杀死细胞,事实上,结合位点很快就会再生。位点破坏活性与P22头部反应产生活性噬菌体,并与针对纯化噬菌体制备的抗血清反应。因此,位点破坏活性是基板部分的一个特征,而不是制剂中的某种污染物。