Parmacek M S, Fox J H, Harrison W H, Garron D C, Swenie D
Gerontology. 1979;25(4):185-91. doi: 10.1159/000212339.
Brain slices of mice (strain CBF1) were used to study the effect of aging on cerebral cortex respiration and metabolism. Young animals (average age 6 months) were compared with old animals (average age 34 months). Metabolism was measured at a normal temperature (37 degrees C) and under hyperthermic stress (40 degrees C). The brain slices were incubated with 14C-glucose under standard conditions with the following parameters being measured: oxygen uptake, 14CO2 production, glucose utilization, and lactate and pyruvate formation. At the normal temperature, there were significant age-associated decreases in oxygen uptake and 14CO2 production but the other parameters were unchanged. At hyperthermic conditions there were significant age-associated decreases in oxygen uptake, 14CO2 production, lactate production, and glucose utilization. Also, in the hyperthermia study, all values were raised from control study values (37 degrees C) with old animals showing smaller increases in glucose utilization and lactate formation. These findings indicate the dysfunction of a number of metabolic pathways in the aged animal.
使用小鼠（CBF1品系）的脑片来研究衰老对大脑皮层呼吸和代谢的影响。将年轻动物（平均年龄6个月）与老年动物（平均年龄34个月）进行比较。在正常温度（37摄氏度）和热应激（40摄氏度）条件下测量代谢。在标准条件下用14C-葡萄糖孵育脑片，测量以下参数：氧气摄取、14CO2生成、葡萄糖利用以及乳酸和丙酮酸的形成。在正常温度下，氧气摄取和14CO2生成存在与年龄相关的显著下降，但其他参数未发生变化。在热应激条件下，氧气摄取、14CO2生成、乳酸生成和葡萄糖利用均存在与年龄相关的显著下降。此外，在热应激研究中，所有数值均高于对照研究数值（37摄氏度），老年动物的葡萄糖利用和乳酸形成增加幅度较小。这些发现表明老年动物中多种代谢途径存在功能障碍。
