Wehr C T, Waskell L, Glaser D A
J Bacteriol. 1975 Jan;121(1):99-107. doi: 10.1128/jb.121.1.99-107.1975.
Four cold-sensitive mutants of Escherichia coli have been isolated which show a reduced ability to synthesize deoxyribonucleic acid at low temperature. The mutants also have a reduced ability to incorporate nucleoside triphosphates into deoxyribonucleic acid at low temperature in cell preparations made permeable with toluene. All four mutations are located at or near the dnaA locus on the E. coli genetic map. They are recessive to the wild-type allele and two of them can be integratively suppressed by F episomes.
已分离出大肠杆菌的四个冷敏感突变体,它们在低温下合成脱氧核糖核酸的能力降低。在经甲苯处理变得通透的细胞制剂中,这些突变体在低温下将核苷三磷酸掺入脱氧核糖核酸的能力也降低。所有这四个突变都位于大肠杆菌遗传图谱上的dnaA基因座或其附近。它们对野生型等位基因是隐性的,其中两个可以被F附加体整合抑制。