作为特定生长速率函数的正在合成蛋白质的核糖体比例。
Fraction of ribosomes synthesizing protein as a function of specific growth rate.
作者信息
Harvey R J
出版信息
J Bacteriol. 1973 Apr;114(1):287-93. doi: 10.1128/jb.114.1.287-293.1973.
Abstract
The fraction of ribosomes engaged in protein synthesis in Escherichia coli growing at specific growth rates ranging from 0.3 to 1.2 h(-1) was estimated from measurements of nascent protein/ribosome and of polyribosomes. Both measurements showed that the fraction of ribosomes synthesizing protein increased with specific growth rate, from 30 to 70% over the range studied. Polyribosome measurements made at different specific growth rates in four E. coli strains showed no significant differences between strains. The increase in the fraction of polyribosomes had begun within 30 s after a shift-up from glucose-minimal medium, and was complete within 2 to 5 min. These results indicate that the function of ribosomes is regulated.
摘要
通过对新生蛋白质/核糖体以及多核糖体的测量，估算了在特定生长速率（范围为0.3至1.2 h⁻¹）下生长的大肠杆菌中参与蛋白质合成的核糖体比例。这两项测量均表明，合成蛋白质的核糖体比例随特定生长速率增加，在所研究的范围内从30%增至70%。在四种大肠杆菌菌株中于不同特定生长速率下进行的多核糖体测量显示，各菌株之间无显著差异。从葡萄糖基本培养基向上转换后30秒内，多核糖体比例就已开始增加，并在2至5分钟内完成。这些结果表明核糖体的功能受到调控。
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