不同生长速率下大肠杆菌中的核糖体模式。
Ribosome patterns in Escherichia coli growing at various rates.
作者信息
Varricchio F, Monier R
出版信息
J Bacteriol. 1971 Oct;108(1):105-10. doi: 10.1128/jb.108.1.105-110.1971.
Abstract
The distribution of ribosomes, 30 and 50S subunits and polysomes, at three different growth rates of Escherichia coli strains B and K-12 has been studied. The usual percentage of subunits is about 20%. However, at the lowest growth rate (mu = generations/hour), mu = 0.45 at 30C, the proportion of subunits is about 30%. An exceptional situation exists in K-12 strains growing at maximum growth rate, mu = 1.35, where the percentage of subunits is 45%. Several points of control over ribosome production are thus indicated. It is suggested that "subunit pool" is essentially a reserve. Furthermore, the polysome content when related to deoxyribonucleic acid content varies directly with the growth rate, which indicates the average efficiency of polysomes in protein synthesis does not vary over the range of growth rates tested.
摘要
对大肠杆菌B株和K - 12株在三种不同生长速率下核糖体、30S和50S亚基以及多核糖体的分布进行了研究。亚基的通常比例约为20%。然而，在最低生长速率（μ = 世代/小时）下，30℃时μ = 0.45，亚基的比例约为30%。在以最大生长速率μ = 1.35生长的K - 12菌株中存在一种特殊情况，其中亚基的百分比为45%。由此表明了对核糖体产生的几个控制点。有人提出“亚基库”本质上是一种储备。此外，当多核糖体含量与脱氧核糖核酸含量相关时，它随生长速率直接变化，这表明在测试的生长速率范围内，多核糖体在蛋白质合成中的平均效率没有变化。
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