鼠伤寒沙门氏菌组氨酸操纵子的自我调节阻遏蛋白的分离。
Isolation of the self-regulated repressor protein of the Hut operons of Salmonella typhimurium.
作者信息
Hagen D C, Magasanik B
出版信息
Proc Natl Acad Sci U S A. 1973 Mar;70(3):808-12. doi: 10.1073/pnas.70.3.808.
Abstract
In Salmonella typhimurium the structural genes of the enzymes responsible for histidine utilization (hut) are clustered in two adjacent operons. A single repressor regulates both operons. The repressor itself is a member of one of the hut operons and, thus, regulates its own synthesis. We have assayed the hut repressor by its ability to bind radioactive DNA to nitrocellulose filters. The binding is specific for DNA bearing the hut operons, and the binding is abolished by the inducer, urocanate. As a member of one of the hut operons, the repressor is inducible, subject to catabolite repression, and affected by a promoter mutation.
摘要
在鼠伤寒沙门氏菌中，负责组氨酸利用的酶的结构基因（hut）聚集在两个相邻的操纵子中。单个阻遏物调节这两个操纵子。该阻遏物本身是其中一个hut操纵子的成员，因此调节其自身的合成。我们通过其将放射性DNA结合到硝酸纤维素滤膜上的能力来检测hut阻遏物。这种结合对携带hut操纵子的DNA具有特异性，并且这种结合会被诱导物尿刊酸消除。作为其中一个hut操纵子的成员，该阻遏物是可诱导的，受到分解代谢物阻遏的影响，并受启动子突变的影响。
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本文引用的文献
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Nature and self-regulated synthesis of the repressor of the hut operons in Salmonella typhimurium.鼠伤寒沙门氏菌组氨酸利用操纵子阻遏物的性质及自我调节合成
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1493-7. doi: 10.1073/pnas.68.7.1493.
6
The two operons of the histidine utilization system in Salmonella typhimurium.鼠伤寒沙门氏菌中组氨酸利用系统的两个操纵子。
J Biol Chem. 1971 May 25;246(10):3330-41.
7
Genetic and metabolic control of enzymes responsible for histidine degradation in Salmonella typhimurium. 4-imidazolone-5-propionate amidohydrolase and N-formimino-L-glutamate formiminohydrolase.鼠伤寒沙门氏菌中负责组氨酸降解的酶的遗传和代谢调控。4-咪唑酮-5-丙酸酰胺水解酶和N-甲酰亚氨基-L-谷氨酸甲酰亚氨基水解酶。
J Biol Chem. 1971 May 25;246(10):3320-9.
8
Lac repressor-operator interaction. I. Equilibrium studies.乳糖阻遏蛋白与操纵基因的相互作用。I. 平衡研究。
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9
Genetic and metabolic control of histidase and urocanase in Salmonella typhimurium, strain 15-59.鼠伤寒沙门氏菌15 - 59株中组氨酸酶和尿刊酸酶的遗传与代谢调控
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Genetic control of histidine degradation in Salmonella typhimurium, strain LT-2.鼠伤寒沙门氏菌LT-2菌株中组氨酸降解的遗传控制。
J Biol Chem. 1969 Oct 10;244(19):5382-91.
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