Bloom F R, Streicher S L, Tyler B
J Bacteriol. 1977 Jun;130(3):983-90. doi: 10.1128/jb.130.3.983-990.1977.
In Klebsiella aerogenes but not in Salmonella typhimurium glutamine synthetase can function during nitrogen-limited growth to increase the rate of synthesis of histidase from the hut genes of S. typhimurium 15-59 (hutS. 15-59). Formation of proline oxidase is also not increased in nitrogen-limited cultures of S. typhimurium. However, in hybrid strains of Escherichia coli or K. aerogenes, the glutamine synthetase of S. typhimurium activates synthesis of histidase from the hutS. 15-59 genes. Apparently, glutamine synthetase is necessary but not sufficient for activation of transcription of the hut genes; another factor must also be present. This factor is active in both K. aerogenes and E. coli but is missing or altered in S. typhimurium.
在产气克雷伯菌中，谷氨酰胺合成酶在氮限制生长期间可以发挥作用，以提高鼠伤寒沙门氏菌15 - 59（hutS. 15 - 59）的hut基因所编码的组氨酸酶的合成速率，但在鼠伤寒沙门氏菌中则不行。在鼠伤寒沙门氏菌的氮限制培养物中，脯氨酸氧化酶的形成也没有增加。然而，在大肠杆菌或产气克雷伯菌的杂交菌株中，鼠伤寒沙门氏菌的谷氨酰胺合成酶可激活hutS. 15 - 59基因合成组氨酸酶。显然，谷氨酰胺合成酶对于hut基因转录的激活是必要的，但不是充分的；还必须存在另一个因子。这个因子在产气克雷伯菌和大肠杆菌中都有活性，但在鼠伤寒沙门氏菌中缺失或发生了改变。
