Rotter V, Globerson A, Nakamura I, Trainin N
J Exp Med. 1973 Jul 1;138(1):130-42. doi: 10.1084/jem.138.1.130.
The immune response to SRBC was measured in the spleens of adult thymectomized, total body irradiated mice injected with various combinations of thymus and bone marrow cells together with thymic humoral factor (THF). It was found that the number of plaque-forming cells was significantly increased when THF was given in vivo immediately after thymus cell administration or when thymus cells were incubated in THF before injection. On the other hand, bone marrow cells equally treated did not manifest any T cell activity, since THF-treated bone marrow cells were not able to substitute thymus cells in the system used. The results accumulated in the present experiments indicate, therefore, that the target cells for THF activity are thymus cells which acquire a higher T helper cell capacity after THF treatment.
在成年胸腺切除、全身照射的小鼠脾脏中，检测对绵羊红细胞（SRBC）的免疫反应。这些小鼠注射了胸腺细胞、骨髓细胞与胸腺体液因子（THF）的各种组合。结果发现，在给予胸腺细胞后立即在体内给予THF，或者在注射前将胸腺细胞在THF中孵育时，噬斑形成细胞的数量显著增加。另一方面，经过同样处理的骨髓细胞未表现出任何T细胞活性，因为在所用系统中，经THF处理的骨髓细胞无法替代胸腺细胞。因此，本实验积累的结果表明，THF活性的靶细胞是胸腺细胞，其在THF处理后获得更高的T辅助细胞能力。
