微管蛋白与磷脂囊泡的相互作用。
Interaction of microtubule proteins with phospholipid vesicles.
作者信息
Caron J M, Berlin R D
出版信息
J Cell Biol. 1979 Jun;81(3):665-71. doi: 10.1083/jcb.81.3.665.
Abstract
We have examined the interaction of unilamellar dimyristoyl phosphatidylcholine liposomes with the high-speed supernate of brain homogenate and with tubulin purified through one or two cycles of microtubule assembly-disassembly. Tubulin and certian high molecular weight proteins are selectively adsorbed from these mixtures onto liposomes. The composition of adsorbed proteins is similar to that obtained during corresponding cycles of microtubule assembly, suggesting the equivalency of these processes. Adsorption induces stacking and/or fusion of liposomes into multilamellar structures indicating strong protein-lipid interaction. In addition, liposome-adsorbed tubulin forms extensive intermolecular disulfide bridges that are inert to reducing agents in the aqueous medium. The observations form a basis for further study of the distribution, function, and properties of membrane-bound tubulin.
摘要
我们研究了单层二肉豆蔻酰磷脂酰胆碱脂质体与脑匀浆高速上清液以及通过一两个微管组装-拆卸循环纯化的微管蛋白之间的相互作用。微管蛋白和某些高分子量蛋白质从这些混合物中选择性地吸附到脂质体上。吸附蛋白质的组成与在微管组装的相应循环中获得的组成相似，表明这些过程是等效的。吸附诱导脂质体堆叠和/或融合成多层结构，表明蛋白质-脂质之间有强烈的相互作用。此外，脂质体吸附的微管蛋白形成广泛的分子间二硫键，这些二硫键在水性介质中对还原剂呈惰性。这些观察结果为进一步研究膜结合微管蛋白的分布、功能和特性奠定了基础。
相似文献
1
Interaction of microtubule proteins with phospholipid vesicles.微管蛋白与磷脂囊泡的相互作用。
J Cell Biol. 1979 Jun;81(3):665-71. doi: 10.1083/jcb.81.3.665.
2
Dynamic interactions between microtubules and artificial membranes.微管与人工膜之间的动态相互作用。
Biochemistry. 1987 Jun 16;26(12):3681-8. doi: 10.1021/bi00386a063.
3
Phase transitions in phospholipid model membranes of different curvature.不同曲率的磷脂模型膜中的相变。
Biochim Biophys Acta. 1978 Jan 19;506(2):183-91. doi: 10.1016/0005-2736(78)90389-9.
4
Stacking in lipid vesicle-tubulin mixtures is an artifact of negative staining.脂质囊泡-微管蛋白混合物中的堆积是负染色的假象。
J Cell Biol. 1980 Sep;86(3):881-4. doi: 10.1083/jcb.86.3.881.
5
Interaction of phospholipid vesicles with cells. Endocytosis and fusion as alternate mechanisms for the uptake of lipid-soluble and water-soluble molecules.磷脂囊泡与细胞的相互作用。内吞作用和融合作为摄取脂溶性分子和水溶性分子的两种替代机制。
J Cell Biol. 1975 Sep;66(3):621-34. doi: 10.1083/jcb.66.3.621.
6
Interaction of tubulin with phospholipid vesicles. I. Association with vesicles at the phase transition.微管蛋白与磷脂囊泡的相互作用。I. 在相变时与囊泡的结合
J Biol Chem. 1981 Jun 10;256(11):5879-85.
7
RPE65, the major retinal pigment epithelium microsomal membrane protein, associates with phospholipid liposomes.视网膜色素上皮特异性65kDa蛋白（RPE65），即主要的视网膜色素上皮微粒体膜蛋白，可与磷脂脂质体结合。
Arch Biochem Biophys. 1997 Oct 1;346(1):21-7. doi: 10.1006/abbi.1997.0276.
8
Interaction of tubulin with phospholipid vesicles. II. Physical changes of the protein.微管蛋白与磷脂囊泡的相互作用。II. 蛋白质的物理变化。
J Biol Chem. 1981 Jun 10;256(11):5886-9.
9
Assembly properties and applications of a new exopolymeric compound excreted by Pseudoalteromonas antarctica NF3.
J Biomater Sci Polym Ed. 2001;12(4):409-27. doi: 10.1163/156856201750195298.
10
Substance P: binding to unilamellar and multilamellar liposomes made from mixtures of phosphatidylcholine and phosphatidic acid.
Pharmazie. 1984 Nov;39(11):765-9.
引用本文的文献
1
Global and local effects in lipid-mediated interactions between peripheral and integral membrane proteins.外周膜蛋白与整合膜蛋白之间脂质介导相互作用中的全局和局部效应。
Front Mol Biosci. 2025 May 30;12:1605772. doi: 10.3389/fmolb.2025.1605772. eCollection 2025.
2
Dimeric Tubulin Modifies Mechanical Properties of Lipid Bilayer, as Probed Using Gramicidin A Channel.二聚体微管蛋白改变脂双层的力学性质，如用短杆菌肽 A 通道探测到的那样。
Int J Mol Sci. 2024 Feb 12;25(4):2204. doi: 10.3390/ijms25042204.
3
Structural features and lipid binding domain of tubulin on biomimetic mitochondrial membranes.仿生线粒体膜上微管蛋白的结构特征与脂质结合结构域
Proc Natl Acad Sci U S A. 2017 May 2;114(18):E3622-E3631. doi: 10.1073/pnas.1619806114. Epub 2017 Apr 18.
4
Tau binds to lipid membrane surfaces via short amphipathic helices located in its microtubule-binding repeats.Tau通过位于其微管结合重复序列中的短两性螺旋与脂质膜表面结合。
Biophys J. 2014 Sep 16;107(6):1441-52. doi: 10.1016/j.bpj.2014.07.046.
5
Shedding of phosphatidylserine from developing erythroid cells involves microtubule depolymerization and affects membrane lipid composition.发育中的红细胞从细胞膜上排出磷脂酰丝氨酸涉及微管的去聚合作用，并影响膜脂组成。
J Membr Biol. 2012 Dec;245(12):779-87. doi: 10.1007/s00232-012-9478-7. Epub 2012 Jul 24.
6
Membrane lipid composition regulates tubulin interaction with mitochondrial voltage-dependent anion channel.膜脂组成调节微管蛋白与线粒体电压依赖性阴离子通道的相互作用。
J Biol Chem. 2012 Aug 24;287(35):29589-98. doi: 10.1074/jbc.M112.378778. Epub 2012 Jul 4.
7
Mechanisms of tubulin modification by phosphatidylcholine hydroperoxides.磷脂酰胆碱氢过氧化物对微管蛋白的修饰机制。
Lipids. 2000 Feb;35(2):205-11. doi: 10.1007/BF02664771.
8
Posttranslational modification of tubulin by palmitoylation: I. In vivo and cell-free studies.微管蛋白经棕榈酰化的翻译后修饰：I. 体内及无细胞体系研究
Mol Biol Cell. 1997 Apr;8(4):621-36. doi: 10.1091/mbc.8.4.621.
9
Phosphorylation in vivo of chick brain microtubule-associated phospholipids.鸡脑微管相关磷脂的体内磷酸化作用
Neurochem Res. 1996 Sep;21(9):1097-103. doi: 10.1007/BF02532420.
10
Cytoplasmic microtubules and fungal morphogenesis: ultrastructural effects of methyl benzimidazole-2-ylcarbamate determined by freeze-substitution of hyphal tip cells.细胞质微管与真菌形态发生：通过菌丝尖端细胞的冷冻置换法确定的甲基苯并咪唑-2-基氨基甲酸酯的超微结构效应
J Cell Biol. 1980 Oct;87(1):55-64. doi: 10.1083/jcb.87.1.55.
本文引用的文献
1
Intermolecular disulfide bonds at central nervous system synaptic junctions.中枢神经系统突触连接处的分子间二硫键。
Biochem Biophys Res Commun. 1976 Dec 20;73(4):858-64. doi: 10.1016/0006-291x(76)90200-x.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
PROTEOLIPIDS. IV. FORMATION OF COMPLEXES BETWEEN CYTOCHROME C AND PURIFIED PHOSPHOLIPIDS.蛋白脂质。IV. 细胞色素C与纯化磷脂之间复合物的形成
Biochemistry. 1965 May;4:859-65. doi: 10.1021/bi00881a010.
4
5
The binding of serum albumin to phospholipid liposomes.血清白蛋白与磷脂脂质体的结合。
Biochim Biophys Acta. 1970 Dec 1;219(2):253-62. doi: 10.1016/0005-2736(70)90204-x.
6
Colchicine-binding activity in particulate fractions of mouse brain.小鼠脑组织微粒体部分中的秋水仙碱结合活性。
J Neurochem. 1970 Sep;17(9):1355-64. doi: 10.1111/j.1471-4159.1970.tb06870.x.
7
Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes.高结合比例下十二烷基硫酸盐与蛋白质的结合。对生物膜中蛋白质状态的可能影响。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):1002-7. doi: 10.1073/pnas.66.3.1002.
8
Microtubule protein. Identification in and transport to nerve endings.微管蛋白。在神经末梢中的鉴定及向神经末梢的转运。
J Cell Biol. 1971 Oct;51(1):138-47. doi: 10.1083/jcb.51.1.138.
9
Effect of colchicine and vinblastine on the topographical separation of membrane functions.秋水仙碱和长春碱对膜功能区域分离的影响。
J Exp Med. 1972 Jul 1;136(1):1-7. doi: 10.1084/jem.136.1.1.
10
Muscle-like contractile proteins and tubulin in synaptosomes.突触小体中的肌肉样收缩蛋白和微管蛋白。
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4472-6. doi: 10.1073/pnas.71.11.4472.
Zotero 插件