鼠伤寒沙门氏菌LT2在以乙酸盐或长链脂肪酸为碳源生长时，异亮氨酸和缬氨酸的生物合成需要乙酰羟酸合酶I。
Acetohydroxy acid synthase I is required for isoleucine and valine biosynthesis by Salmonella typhimurium LT2 during growth on acetate or long-chain fatty acids.
作者信息
Dailey F E, Cronan J E, Maloy S R
出版信息
J Bacteriol. 1987 Feb;169(2):917-9. doi: 10.1128/jb.169.2.917-919.1987.
Abstract
Salmonella typhimurium LT2 normally expresses two acetohydroxy acid synthases (AHAS I and AHAS II). The function of AHAS I in this organism was unclear, since AHAS I-deficient (ilvBN) mutants of LT2 grew well on glucose or succinate minimal media, whereas AHAS II-deficient (ilvGM) mutants requried isoleucine for normal growth on glucose minimal media. We report that AHAS I-deficient mutants of S. typhimurium required isoleucine and valine for growth on acetate or oleate minimal media, whereas AHAS II-deficient mutants were able to grow on these media without isoleucine supplementation.
摘要
鼠伤寒沙门氏菌LT2通常表达两种乙酰羟酸合酶（AHAS I和AHAS II）。AHAS I在该生物体中的功能尚不清楚，因为LT2的AHAS I缺陷型（ilvBN）突变体在葡萄糖或琥珀酸盐基本培养基上生长良好，而AHAS II缺陷型（ilvGM）突变体在葡萄糖基本培养基上正常生长需要异亮氨酸。我们报告称，鼠伤寒沙门氏菌的AHAS I缺陷型突变体在乙酸盐或油酸盐基本培养基上生长需要异亮氨酸和缬氨酸，而AHAS II缺陷型突变体在不添加异亮氨酸的情况下能够在这些培养基上生长。
相似文献
1
Acetohydroxy acid synthase I is required for isoleucine and valine biosynthesis by Salmonella typhimurium LT2 during growth on acetate or long-chain fatty acids.鼠伤寒沙门氏菌LT2在以乙酸盐或长链脂肪酸为碳源生长时，异亮氨酸和缬氨酸的生物合成需要乙酰羟酸合酶I。
J Bacteriol. 1987 Feb;169(2):917-9. doi: 10.1128/jb.169.2.917-919.1987.
2
Acetohydroxy acid synthase I, a required enzyme for isoleucine and valine biosynthesis in Escherichia coli K-12 during growth on acetate as the sole carbon source.乙酰羟酸合酶I，是大肠杆菌K-12在以乙酸盐作为唯一碳源生长期间异亮氨酸和缬氨酸生物合成所需的一种酶。
J Bacteriol. 1986 Feb;165(2):453-60. doi: 10.1128/jb.165.2.453-460.1986.
3
Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II.鼠伤寒沙门氏菌中乙酰羟酸合酶I和II缺陷型突变体。
J Bacteriol. 1980 Mar;141(3):1258-63. doi: 10.1128/jb.141.3.1258-1263.1980.
4
Evidence for isoleucine as a positive effector of the ilvBN operon in Salmonella typhimurium.异亮氨酸作为鼠伤寒沙门氏菌中ilvBN操纵子的正效应物的证据。
Biochem Biophys Res Commun. 1991 Aug 15;178(3):934-9. doi: 10.1016/0006-291x(91)90981-c.
5
Acetohydroxy acid synthase isoenzymes of Escherichia coli K12 and Salmonella typhimurium.大肠杆菌K12和鼠伤寒沙门氏菌的乙酰羟酸合酶同工酶。
Ann Microbiol (Paris). 1982 Mar-Apr;133(2):251-6.
6
Physiological implications of the specificity of acetohydroxy acid synthase isozymes of enteric bacteria.肠道细菌乙酰羟酸合酶同工酶特异性的生理学意义。
J Bacteriol. 1987 Aug;169(8):3750-6. doi: 10.1128/jb.169.8.3750-3756.1987.
7
Acetohydroxy acid synthase and threonine deaminase activities, and the biosynthesis of isoleucine-leucine-valine in Streptococcus bovis.牛链球菌中乙酰羟酸合酶和苏氨酸脱氨酶的活性以及异亮氨酸-亮氨酸-缬氨酸的生物合成
Res Microbiol. 1993 Sep;144(7):539-45. doi: 10.1016/0923-2508(93)90003-k.
8
Separate regulation of transport and biosynthesis of leucine, isoleucine, and valine in bacteria.细菌中亮氨酸、异亮氨酸和缬氨酸转运与生物合成的独立调控。
J Bacteriol. 1975 Jun;122(3):994-1000. doi: 10.1128/jb.122.3.994-1000.1975.
9
Substrate channeling: alpha-ketobutyrate inhibition of acetohydroxy acid synthase in Salmonella typhimurium.底物通道化：α-酮丁酸对鼠伤寒沙门氏菌乙酰羟酸合酶的抑制作用
J Bacteriol. 1980 Sep;143(3):1509-12. doi: 10.1128/jb.143.3.1509-1512.1980.
10
Biosynthesis of branched-chain amino acids in Schizosaccharomyces pombe: properties of acetohydroxy acid synthetase.粟酒裂殖酵母中支链氨基酸的生物合成：乙酰羟酸合成酶的特性
J Bacteriol. 1973 Apr;114(1):332-40. doi: 10.1128/jb.114.1.332-340.1973.
引用本文的文献
1
Conversion of Escherichia coli pyruvate oxidase to an 'alpha-ketobutyrate oxidase'.将大肠杆菌丙酮酸氧化酶转化为“α-酮丁酸氧化酶”。
Biochem J. 2000 Dec 15;352 Pt 3(Pt 3):717-24.
2
Branched-chain amino acid biosynthesis in Salmonella typhimurium: a quantitative analysis.鼠伤寒沙门氏菌中支链氨基酸的生物合成：定量分析。
J Bacteriol. 1998 Aug;180(16):4056-67. doi: 10.1128/JB.180.16.4056-4067.1998.
3
Isolation and characterization of Salmonella typhimurium glyoxylate shunt mutants.鼠伤寒沙门氏菌乙醛酸循环突变体的分离与鉴定。
J Bacteriol. 1987 Jul;169(7):3029-34. doi: 10.1128/jb.169.7.3029-3034.1987.
4
Linkage map of Salmonella typhimurium, edition VII.鼠伤寒沙门氏菌连锁图谱，第七版。
Microbiol Rev. 1988 Dec;52(4):485-532. doi: 10.1128/mr.52.4.485-532.1988.
5
Physiological implications of the substrate specificities of acetohydroxy acid synthases from varied organisms.来自不同生物体的乙酰羟酸合酶底物特异性的生理学意义。
J Bacteriol. 1990 Jun;172(6):3444-9. doi: 10.1128/jb.172.6.3444-3449.1990.
6
Properties of subcloned subunits of bacterial acetohydroxy acid synthases.细菌乙酰羟酸合酶亚克隆亚基的特性
J Bacteriol. 1992 Sep;174(17):5560-6. doi: 10.1128/jb.174.17.5560-5566.1992.
本文引用的文献
1
Metabolic basis for the isoleucine, pantothenate or methionine requirement of ilvG strains of Salmonella typhimurium.鼠伤寒沙门氏菌ilvG菌株对异亮氨酸、泛酸盐或蛋氨酸需求的代谢基础。
J Bacteriol. 1982 Jun;150(3):1202-11. doi: 10.1128/jb.150.3.1202-1211.1982.
2
Plasmid insertion mutagenesis and lac gene fusion with mini-mu bacteriophage transposons.利用微小噬菌体转座子进行质粒插入诱变和乳糖操纵子基因融合
J Bacteriol. 1984 May;158(2):488-95. doi: 10.1128/jb.158.2.488-495.1984.
3
Regulation of cyclic AMP of the ilvB-encoded biosynthetic acetohydroxy acid synthase in Escherichia coli K-12.大肠杆菌K-12中ilvB编码的生物合成乙酰羟酸合酶的环磷酸腺苷调节
Mol Gen Genet. 1980 Apr;178(1):179-83. doi: 10.1007/BF00267227.
4
Salmonella typhimurium mutants defective in acetohydroxy acid synthases I and II.鼠伤寒沙门氏菌中乙酰羟酸合酶I和II缺陷型突变体。
J Bacteriol. 1980 Mar;141(3):1258-63. doi: 10.1128/jb.141.3.1258-1263.1980.
5
IlvHI locus of Salmonella typhimurium.鼠伤寒沙门氏菌的ilvHI基因座。
J Bacteriol. 1983 Jun;154(3):1054-63. doi: 10.1128/jb.154.3.1054-1063.1983.
6
Regulation of expression of the ilvB operon in Salmonella typhimurium.鼠伤寒沙门氏菌中ilvB操纵子表达的调控
J Bacteriol. 1984 Dec;160(3):833-41. doi: 10.1128/jb.160.3.833-841.1984.
7
Culture medium for enterobacteria.用于肠道细菌的培养基。
J Bacteriol. 1974 Sep;119(3):736-47. doi: 10.1128/jb.119.3.736-747.1974.
8
Temperature-sensitive growth inhibition by valine in Salmonella typhimurium: alteration of one form of acetohydroxy acid synthetase.鼠伤寒沙门氏菌中缬氨酸对温度敏感的生长抑制作用：一种乙酰羟酸合成酶形式的改变
J Bacteriol. 1973 Oct;116(1):98-106. doi: 10.1128/jb.116.1.98-106.1973.
9
The effect of carbon and nitrogen sources on the level of metabolic intermediates in Escherichia coli.碳源和氮源对大肠杆菌中代谢中间体水平的影响。
J Biol Chem. 1971 Nov;246(21):6511-21.
10
Purification and properties of Salmonella typhimurium acetolactate synthase isozyme II from Escherichia coli HB101/pDU9.鼠伤寒沙门氏菌乙酰乳酸合酶同工酶II从大肠杆菌HB101/pDU9中的纯化及性质
Biochemistry. 1985 Aug 27;24(18):4952-9. doi: 10.1021/bi00339a034.
Zotero 插件