Tilby M, Hindennach I, Henning U
J Bacteriol. 1978 Dec;136(3):1189-91. doi: 10.1128/jb.136.3.1189-1191.1978.
Colicin E3 was found to kill, under conditions of osmotic shock, cells lacking a functional outer membrane receptor (bfe). Under such conditions, component A of the colicin, carrying endonucleolytic activity, also killed bfe cells, whereas fragment T2, obtained by tryptic digestion of the colicin and also active endonucleolytically, was inactive. Tolerance to the colicin caused by defects in the outer membrane could be overcome by osmotic shock, whereas tolerance probably caused by an altered plasma membrane could not.
发现在渗透休克条件下，大肠杆菌素E3可杀死缺乏功能性外膜受体（bfe）的细胞。在这种条件下，具有核酸内切酶活性的大肠杆菌素组分A也能杀死bfe细胞，而通过胰蛋白酶消化大肠杆菌素获得的同样具有核酸内切酶活性的片段T2却没有活性。外膜缺陷导致的对大肠杆菌素的耐受性可通过渗透休克克服，而可能由质膜改变引起的耐受性则不能。
