Elliott J, Richter C, Souther A, Bruner R
J Virol. 1973 Dec;12(6):1253-8. doi: 10.1128/JVI.12.6.1253-1258.1973.
We investigated the synthesis of DNA in toluene-treated cells prepared from Escherichia coli infected with bacteriophage T4. If the phage carry certain rII deletion mutations, those which extend into the nearby D2a region, the following results are obtained: (i) phage DNA synthesis occurs unless the phage carries certain DNA-negative mutations; and (ii) host DNA synthesis occurs even though the phage infection has already resulted in the cessation of host DNA synthesis in vivo. The latter result indicates that the phage-induced cessation of host DNA synthesis is not due to an irreversible inactivation of an essential component of the replication apparatus. If the phage are D2a(+), host DNA synthesis in toluene-treated infected cells is markedly reduced; phage DNA synthesis is probably also reduced somewhat. These D2a effects, considered along with our earlier work, suggest that a D2a-controlled nuclease, specific for cytosine-containing DNA, is active in toluene-treated cells.
我们研究了从感染噬菌体T4的大肠杆菌制备的经甲苯处理的细胞中DNA的合成情况。如果噬菌体携带某些rII缺失突变，即那些延伸到附近D2a区域的突变，会得到以下结果：（i）除非噬菌体携带某些DNA阴性突变，否则会发生噬菌体DNA合成；（ii）即使噬菌体感染在体内已经导致宿主DNA合成停止，宿主DNA合成仍会发生。后一个结果表明，噬菌体诱导的宿主DNA合成停止并非由于复制装置的关键成分发生不可逆失活。如果噬菌体是D2a（+），经甲苯处理的感染细胞中的宿主DNA合成会显著减少；噬菌体DNA合成可能也会有所减少。结合我们早期的工作来考虑这些D2a效应，表明一种对含胞嘧啶的DNA具有特异性的D2a控制的核酸酶在经甲苯处理的细胞中具有活性。
