Kane S M, Roth R
J Bacteriol. 1974 Apr;118(1):8-14. doi: 10.1128/jb.118.1.8-14.1974.
Carbohydrate metabolism, under sporulation conditions, was compared in sporulating and non-sporulating diploids of Saccharomyces cerevisiae. Total carbohydrate was fractionated into trehalose, glycogen, mannan, and an alkali-insoluble fraction composed of glucan and insoluble glycogen. The behavior of three fractions was essentially the same in both sporulating and non-sporulating strains; trehalose, mannan, and the insoluble fraction were all synthesized to about the same extent regardless of a strain's ability to undergo meiosis or sporulation. In contrast, aspects of soluble glycogen metabolism depended on sporulation. Although glycogen synthesis took place in both sporulating and non-sporulating strains, only sporulating strains exhibited a period of glycogen degradation, which coincided with the final maturation of ascospores. We also determined the carbohydrate composition of spores isolated from mature asci. Spores contained all components present in vegetative cells, but in different proportions. In cells, the most abundant carbohydrate was mannan, followed by glycogen, then trehalose, and finally the alkali-insoluble fraction; in spores, trehalose was most abundant, followed by the alkali-insoluble fraction, glycogen, and mannan in that order.
在产孢条件下，对酿酒酵母的产孢二倍体和非产孢二倍体的碳水化合物代谢进行了比较。总碳水化合物被分为海藻糖、糖原、甘露聚糖以及由葡聚糖和不溶性糖原组成的碱不溶性部分。在产孢菌株和非产孢菌株中，三种组分的行为基本相同；无论菌株进行减数分裂或产孢的能力如何，海藻糖、甘露聚糖和不溶性部分的合成程度大致相同。相比之下，可溶性糖原代谢的各个方面取决于产孢情况。虽然糖原合成在产孢菌株和非产孢菌株中均有发生，但只有产孢菌株表现出一段糖原降解期，这与子囊孢子的最终成熟相吻合。我们还测定了从成熟子囊中分离出的孢子的碳水化合物组成。孢子含有营养细胞中存在的所有成分，但比例不同。在细胞中，含量最丰富的碳水化合物是甘露聚糖，其次是糖原，然后是海藻糖，最后是碱不溶性部分；在孢子中，海藻糖含量最丰富，其次是碱不溶性部分、糖原，最后是甘露聚糖，顺序依次为上述顺序。
