在酵母孢子形成和营养生长期间从酵母中分离多核糖体。
Isolation of polyribosomes from yeast during sporulation and vegetative growth.
作者信息
Mills D
出版信息
Appl Microbiol. 1974 May;27(5):944-8. doi: 10.1128/am.27.5.944-948.1974.
Abstract
Exponentially growing and sporulating cells of Saccharomyces cerevisiae have been subjected to a variety of conditions which mechanically disrupt the cell in an effort to establish conditions which permit the recovery of intact polyribosomes. Grinding cells for 10 s with glass beads in a Bronwill cell homogenizer was sufficiently gentle to yield a polyribosome content in exponentially growing cells which was similar to values obtained from yeast spheroplasts. Polyribosome patterns in sporulating yeast were similar to those from exponentially growing cells. This technique is fast, reproducible over a wide range of cell concentrations, and eliminates the need to make spheroplasts to recover intact polyribosomes.
摘要
酿酒酵母的指数生长期细胞和孢子形成期细胞已被置于各种条件下，这些条件会机械破坏细胞，以建立能够回收完整多核糖体的条件。在布朗威尔细胞匀浆器中用玻璃珠研磨细胞10秒，这种方法足够温和，能使指数生长期细胞中的多核糖体含量与从酵母原生质体获得的值相似。孢子形成期酵母中的多核糖体模式与指数生长期细胞中的相似。该技术速度快，在广泛的细胞浓度范围内具有可重复性，并且无需制备原生质体即可回收完整的多核糖体。
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