酿酒酵母中核糖核酸和蛋白质的细胞含量与指数生长速率的关系：表观肽链延伸速率的计算
Cellular content of ribonucleic acid and protein in Saccharomyces cerevisiae as a function of exponential growth rate: calculation of the apparent peptide chain elongation rate.
作者信息
Boehlke K W, Friesen J D
出版信息
J Bacteriol. 1975 Feb;121(2):429-33. doi: 10.1128/jb.121.2.429-433.1975.
Abstract
The average cellular content of ribonucleic acid and protein was determined in cultures of Saccharomyces cerevisiae growing exponentially at different rates in a variety of media. Estimations of the proportion of total cellular ribonucleic acid that is made up of ribosomal ribonucleic acid were used to calculate the average number of ribosomes per cell at the different growth rates. The fraction of ribosomes actively engaged in translation was estimated by sucrose gradient centrifugation of ribosomes and polysomes. These data were used in a calculation of the apparent time taken for the addition of an amino acid to the growing polypeptide chain; this value was found to vary linearly with growth rate over a fivefold range of doubling times.
摘要
测定了酿酒酵母在多种培养基中以不同速率指数生长的培养物中核糖核酸和蛋白质的平均细胞含量。通过对构成核糖体核糖核酸的总细胞核糖核酸比例的估计，计算出不同生长速率下每个细胞的平均核糖体数量。通过对核糖体和多核糖体进行蔗糖梯度离心，估计了积极参与翻译的核糖体比例。这些数据用于计算向生长中的多肽链添加一个氨基酸所需的表观时间；发现在五倍的倍增时间范围内，该值随生长速率呈线性变化。
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