一种具有改变的50S核糖体亚基的新型大肠杆菌松弛突变体。
A new relaxed mutant of Escherichia coli with an altered 50S ribosomal subunit.
作者信息
Friesen J D, Fiil N P, Parker J M, Haseltine W A
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3465-9. doi: 10.1073/pnas.71.9.3465.
Abstract
A new relaxed mutant called rel C has been isolated from a rel A(+)/rel A(+) partial diploid strain. The rel C mutant is unable to synthesize ppGpp or pppGpp in vivo in response to amino acid starvation or in vitro, but can synthesize these nucleotides in a shift-down. Rel C maps near rif. Studies in vitro demonstrate the lesion to be probably in one of the 50S ribosomal proteins that can be removed by 1.0 M LiCl.
摘要
一种名为rel C的新型松弛突变体已从rel A(+)/rel A(+)部分二倍体菌株中分离出来。rel C突变体在体内对氨基酸饥饿无反应时或在体外均无法合成ppGpp或pppGpp，但在营养条件降低时能够合成这些核苷酸。Rel C基因座位于rif附近。体外研究表明，该损伤可能存在于一种可被1.0 M LiCl去除的50S核糖体蛋白中。
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