Goyette M, Petropoulos C J, Shank P R, Fausto N
Mol Cell Biol. 1984 Aug;4(8):1493-8. doi: 10.1128/mcb.4.8.1493-1498.1984.
We examined the transcription of six cellular oncogenes during the process of compensatory growth in rat liver after partial hepatectomy. We have previously reported that transcripts of c-rasH are elevated during regenerative growth of the liver. We now report that transcripts of c-rasK and c-myc genes are significantly elevated after partial hepatectomy, whereas transcripts of c-abl and c-src are essentially unchanged and transcripts of c-mos are undetectable in either normal or regenerating rat liver. In liver regeneration after partial hepatectomy or chemical injury, changes in c-myc transcripts occur before DNA synthesis. The elevation of c-myc and c-ras transcripts is sequential in that highest levels of c-myc transcripts were detected 12 to 18 h after partial hepatectomy, whereas the levels of c-rasH and c-rasK were maximal by 36 to 48 h. Transcripts of all three activated oncogenes returned to their basal levels by 96 h.
我们研究了大鼠部分肝切除术后肝脏代偿性生长过程中六个细胞癌基因的转录情况。我们之前曾报道，在肝脏再生性生长过程中，c-rasH的转录本会升高。我们现在报道，部分肝切除术后，c-rasK和c-myc基因的转录本显著升高，而c-abl和c-src的转录本基本未变，且在正常或再生的大鼠肝脏中均未检测到c-mos的转录本。在部分肝切除或化学损伤后的肝脏再生过程中，c-myc转录本的变化发生在DNA合成之前。c-myc和c-ras转录本的升高是有顺序的，即部分肝切除术后12至18小时检测到c-myc转录本的最高水平，而c-rasH和c-rasK的水平在36至48小时达到最大值。所有三个激活的癌基因的转录本在96小时时恢复到基础水平。
