携带R因子的大肠杆菌对庆大霉素、妥布霉素和卡那霉素的3-N酶促乙酰化作用
3-N enzymatic acetylation of gentamicin, tobramycin, and kanamycin by Escherichia coli carrying an R factor.
作者信息
Le Goffic F, Martel A, Witchitz J
出版信息
Antimicrob Agents Chemother. 1974 Dec;6(6):680-4. doi: 10.1128/AAC.6.6.680.
Abstract
A new R factor-mediated gentamicin acetyltransferase obtained from a clinical isolate of gentamicin-, tobramycin-, and kanamycin-resistant Klebsiella was characterized. R factor R 176 was transferred to Escherichia coli K-12 LA 290 from a Klebsiella. Resistance transfer, minimal inhibitory concentrations of various aminoglycoside antibiotics, enzymatic inactivation, and the isoelectric point of the enzyme obtained were determined. We found that R factor R 176 could govern the enzymatic 3-N acetylation of gentamicin, tobramycin, and kanamycin, and isoelectric point determinations suggested that the enzymes governed by R 176 and R 135 are different. The genes responsible for resistance to gentamicin, tobramycin, and kanamycin govern the synthesis of an isoenzyme of AAC(3)I, which we propose to call AAC(3)II.
摘要
对从一株对庆大霉素、妥布霉素和卡那霉素耐药的肺炎克雷伯菌临床分离株中获得的一种新的R因子介导的庆大霉素乙酰转移酶进行了特性鉴定。R因子R 176从肺炎克雷伯菌转移至大肠杆菌K-12 LA 290。测定了耐药性转移、各种氨基糖苷类抗生素的最低抑菌浓度、酶失活情况以及所获得酶的等电点。我们发现R因子R 176可控制庆大霉素、妥布霉素和卡那霉素的3-N-乙酰化酶促反应，等电点测定表明由R 176和R 135控制的酶不同。负责对庆大霉素、妥布霉素和卡那霉素耐药的基因控制着AAC(3)I同工酶的合成，我们建议将其称为AAC(3)II。
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