Hurst A, Kruse H
Antimicrob Agents Chemother. 1972 Mar;1(3):277-9. doi: 10.1128/AAC.1.3.277.
The effect of secondary metabolites added to cultures of the organisms producing them was investigated. Nisin was added to growing cultures of a nisin-producing strain of Streptococcus lactis (354/07) and enterotoxin B to strains of Staphylococcus aureus (S6 and 243) producing enterotoxin B. One quarter (12 mug/ml) of the amount of nisin formed by the culture of S. lactis inhibited lag-phase cells and lysed log-phase cells. The same amount of nisin added before inoculation or at a time when nisin synthesis had started (in late log phase), caused only transient delay in growth. Inhibition of growth of the two enterotoxin B-producing strains of S. aureus could not be demonstrated at any stage of their growth cycle with as much as 1 mg of enterotoxin B per ml of medium.
研究了将次生代谢产物添加到产生这些次生代谢产物的生物体培养物中的效果。将乳酸链球菌素添加到产乳酸链球菌（354/07）的生长培养物中，将肠毒素B添加到产肠毒素B的金黄色葡萄球菌菌株（S6和243）中。乳酸链球菌培养物产生的乳酸链球菌素量的四分之一（12微克/毫升）可抑制迟滞期细胞并裂解对数期细胞。在接种前或乳酸链球菌素合成开始时（对数后期）添加相同量的乳酸链球菌素，只会导致生长的短暂延迟。在每毫升培养基中加入多达1毫克肠毒素B的情况下，在两种产肠毒素B的金黄色葡萄球菌菌株生长周期的任何阶段均未显示出对其生长的抑制作用。
