Lavelle G C, Sturman L, Hadlow W J
Infect Immun. 1972 Mar;5(3):319-23. doi: 10.1128/iai.5.3.319-323.1972.
Spleen cells from mice infected with scrapie virus were separated into subpopulations on the basis of buoyant density in discontinuous gradients of isotonic albumin or differential adherence of cells to plastic. At three different intervals after infection, a population of "less dense" cells was found in albumin gradients that had 40-to 60-fold higher specific infectivity (cells per median lethal dose) than the total cell suspension before gradient sedimentation. The class of cells associated with high relative specific infectivity has a density characteristic of lymphoblasts, myeloblasts, and macrophages. Separation of "macrophage rich" cells on the basis of adherence to plastic did not result in significant enrichment of scrapie virus-infected cells.
将感染瘙痒病病毒的小鼠脾脏细胞，根据其在等渗白蛋白不连续梯度中的浮力密度，或细胞对塑料的差异黏附性，分离成亚群。在感染后的三个不同时间间隔，在白蛋白梯度中发现了一群“密度较低”的细胞，其比梯度沉降前的总细胞悬液具有高40至60倍的比感染性（每半数致死剂量的细胞数）。与高相对比感染性相关的细胞类别具有成淋巴细胞、成髓细胞和巨噬细胞的密度特征。基于对塑料的黏附性分离“富含巨噬细胞”的细胞，并未导致瘙痒病病毒感染细胞的显著富集。
