米氏常数（Km）和抑制常数（Ki）的图形测定
The graphical determination of K m and K i .
作者信息
Dixon M
出版信息
Biochem J. 1972 Aug;129(1):197-202. doi: 10.1042/bj1290197.
Abstract
The principle of Dixon (1965) has been extended to give rapid graphical methods for determining enzyme constants for substrates (K(m)) and inhibitors (K(i)). It does away with the sometimes questionable assumption that the amounts of substrate or inhibitor bound by the enzyme are negligible in comparison with the total amount added, and is therefore valid even for cases of high affinity, where the usual methods fail. Besides doing away with the need for calculation, it enables the concentrations of the various components of the system to be read off directly for any point of the velocity curve.
摘要
狄克逊（1965年）提出的原理已得到扩展，从而能给出快速的图解方法，用于测定底物的酶常数（米氏常数Km）和抑制剂的酶常数（抑制常数Ki）。它摒弃了有时存在问题的假设，即与添加的总量相比，酶结合的底物或抑制剂的量可忽略不计，因此即使在通常方法失效的高亲和力情况下也有效。除了无需计算外，它还能直接读出系统中各组分在速度曲线任意点的浓度。
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本文引用的文献
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