Shultz L D, Wilder M S
Infect Immun. 1973 Feb;7(2):289-97. doi: 10.1128/iai.7.2.289-297.1973.
Viability of Listeria monocytogenes suspended in either normal rabbit serum or listericidal factor is significantly reduced before cell lysis. In normal serum Listeria populations are initially reduced by clumping of viable bacteria, and the cells within these clumps are morphologically altered and killed by the listericidal factor in serum. This clumping is complement independent and does not occur after suspension in purified listericidal factor. In electron micrographs, densely stained amorphous material was seen surrounding clumped cells suspended in serum. Initial damage to Listeria suspended in either serum or listericidal factor appears to be confined to plasma and mesosomal membranes. The mesosomes are rapidly disorganized, and the plasma membrane becomes crenated and separates from the cell wall. Subsequently, the fibrillar organization of the nuclear region disrupts, and the cytoplasm increases in granularity. Studies utilizing Listeria protoplasts provided evidence in support of the plasma membrane as the site of listericidal action. Cell death may be related to loss in enzyme function after crenation of the plasma membrane and mesosomal dissolution.
悬浮于正常兔血清或杀菌因子中的单核细胞增生李斯特菌在细胞裂解前活力显著降低。在正常血清中，李斯特菌数量最初因活细菌聚集而减少，这些菌团内的细胞形态发生改变，并被血清中的杀菌因子杀死。这种聚集不依赖补体，悬浮于纯化的杀菌因子中后不会发生。在电子显微镜照片中，可见密集染色的无定形物质围绕着悬浮于血清中的聚集细胞。悬浮于血清或杀菌因子中的李斯特菌最初的损伤似乎局限于质膜和中体膜。中体迅速解体，质膜出现皱缩并与细胞壁分离。随后，核区的纤维组织被破坏，细胞质颗粒增多。利用李斯特菌原生质体进行的研究为质膜是杀菌作用位点提供了证据。细胞死亡可能与质膜皱缩和中体溶解后酶功能丧失有关。
