Jensen R, Gregory B, Naylor J, Actor P
Infect Immun. 1972 Aug;6(2):156-61. doi: 10.1128/iai.6.2.156-161.1972.
Ribosomal preparations from Vibrio cholerae Ogawa and Inaba are protective immunogens for mice challenged with either serotype. Column chromatography of ribosomal fractions separated protective antigen from the ribosomes. The antigen is a heterogeneous colloid which contains protein, lipid, and carbohydrate in the ratio 3:1:1. Amino acid composition is that of a slightly acidic protein with no unusual complement of amino acids. The lipid component consists of several longchain fatty acids and of phosphatidyl ethanolamine. The major sugars identified were glucose and galactose. The colloid can be dissolved by treatment with chelating agents and sodium dodecyl sulfate. Purification of the solubilized material or of the colloid was unsuccessful.
来自霍乱弧菌小川型和稻叶型的核糖体制剂对用任一血清型攻击的小鼠都是保护性免疫原。核糖体组分的柱色谱法从核糖体中分离出保护性抗原。该抗原是一种异质胶体，其蛋白质、脂质和碳水化合物的比例为3:1:1。氨基酸组成是一种略带酸性的蛋白质，没有异常的氨基酸互补。脂质成分由几种长链脂肪酸和磷脂酰乙醇胺组成。鉴定出的主要糖类是葡萄糖和半乳糖。该胶体可用螯合剂和十二烷基硫酸钠处理溶解。溶解后的物质或胶体的纯化未成功。
