从霍乱弧菌（小川型）核糖体制剂中分离保护性菌体抗原。
Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations.
作者信息
Jensen R, Gregory B, Naylor J, Actor P
出版信息
Infect Immun. 1972 Aug;6(2):156-61. doi: 10.1128/iai.6.2.156-161.1972.
Abstract
Ribosomal preparations from Vibrio cholerae Ogawa and Inaba are protective immunogens for mice challenged with either serotype. Column chromatography of ribosomal fractions separated protective antigen from the ribosomes. The antigen is a heterogeneous colloid which contains protein, lipid, and carbohydrate in the ratio 3:1:1. Amino acid composition is that of a slightly acidic protein with no unusual complement of amino acids. The lipid component consists of several longchain fatty acids and of phosphatidyl ethanolamine. The major sugars identified were glucose and galactose. The colloid can be dissolved by treatment with chelating agents and sodium dodecyl sulfate. Purification of the solubilized material or of the colloid was unsuccessful.
摘要
来自霍乱弧菌小川型和稻叶型的核糖体制剂对用任一血清型攻击的小鼠都是保护性免疫原。核糖体组分的柱色谱法从核糖体中分离出保护性抗原。该抗原是一种异质胶体，其蛋白质、脂质和碳水化合物的比例为3:1:1。氨基酸组成是一种略带酸性的蛋白质，没有异常的氨基酸互补。脂质成分由几种长链脂肪酸和磷脂酰乙醇胺组成。鉴定出的主要糖类是葡萄糖和半乳糖。该胶体可用螯合剂和十二烷基硫酸钠处理溶解。溶解后的物质或胶体的纯化未成功。
相似文献
1
Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations.从霍乱弧菌（小川型）核糖体制剂中分离保护性菌体抗原。
Infect Immun. 1972 Aug;6(2):156-61. doi: 10.1128/iai.6.2.156-161.1972.
2
Biochemical characterization of three mycobacterial ribosomal fractions.三种分枝杆菌核糖体组分的生化特性
Can J Microbiol. 1983 Feb;29(2):185-93. doi: 10.1139/m83-032.
3
4
Antigens of Streptococcus mutans. I. Characterization of a serotype-specific determinant from Streptococcus mutans.变形链球菌的抗原。I. 变形链球菌血清型特异性决定簇的特性
Infect Immun. 1973 May;7(5):795-804. doi: 10.1128/iai.7.5.795-804.1973.
5
Extracellular carbohydrate antigens from some non-pathogenic Neisseria species.来自一些非致病性奈瑟菌属的细胞外碳水化合物抗原。
Can J Microbiol. 1973 Jun;19(6):703-8. doi: 10.1139/m73-114.
6
50-S ribosomal proteins. Purification and partial characterization of two acidic proteins, A 1 and A 2, isolated from 50-S ribosomes of Escherichia coli.50-S核糖体蛋白。从大肠杆菌50-S核糖体中分离出的两种酸性蛋白A1和A2的纯化及部分特性分析。
Eur J Biochem. 1972 Jan 31;25(1):5-12. doi: 10.1111/j.1432-1033.1972.tb01660.x.
7
Chemical composition of a purified membrane fraction from Sarcina aurantiaca in relation to growth phase.与生长阶段相关的金黄色八叠球菌纯化膜组分的化学成分
Antonie Van Leeuwenhoek. 1974;40(1):65-70. doi: 10.1007/BF00394554.
8
Characterization of the antigenic subunits of the envelope protein of Yersinia pestis.鼠疫耶尔森菌包膜蛋白抗原亚基的特性分析
J Bacteriol. 1974 Jan;117(1):48-55. doi: 10.1128/jb.117.1.48-55.1974.
9
Composition and immunochemical properties of outer membrane proteins of Vibrio cholerae.霍乱弧菌外膜蛋白的组成及免疫化学特性
J Bacteriol. 1980 Oct;144(1):382-9. doi: 10.1128/jb.144.1.382-389.1980.
10
Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis.核糖体疫苗。I. 从鼠伤寒沙门氏菌和鼠疫耶尔森氏菌中分离出的核糖体组分的免疫原性。
Infect Immun. 1972 Jun;5(6):947-52. doi: 10.1128/iai.5.6.947-952.1972.
引用本文的文献
1
Immunogenicity of Ribosomal Preparations from Yeast Cells of Histoplasma capsulatum.荚膜组织胞浆菌酵母细胞核糖体制剂的免疫原性。
Infect Immun. 1974 Nov;10(5):1091-7. doi: 10.1128/iai.10.5.1091-1097.1974.
2
Direct evidence for the involvement of capsular polysaccharide in the immunoprotective activity of Klebsiella pneumoniae ribosomal preparations.关于荚膜多糖参与肺炎克雷伯菌核糖体制剂免疫保护活性的直接证据。
Infect Immun. 1981 Jan;31(1):71-7. doi: 10.1128/iai.31.1.71-77.1981.
3
Immunogenicity of ribosomal preparations from Neisseria gonorrhoeae.淋病奈瑟菌核糖体制剂的免疫原性。
Infect Immun. 1980 Apr;28(1):92-100. doi: 10.1128/iai.28.1.92-100.1980.
4
Analysis of cell wall and membrane contamination of ribosomal preparations from Streptococcus mutans.变形链球菌核糖体制剂的细胞壁和膜污染分析
Infect Immun. 1983 Feb;39(2):807-16. doi: 10.1128/iai.39.2.807-816.1983.
5
Immunogenicity of ribosomes from enzymatically lysed Streptococcus pyogenes.经酶解的化脓性链球菌核糖体的免疫原性
Infect Immun. 1980 Feb;27(2):424-30. doi: 10.1128/iai.27.2.424-430.1980.
6
Ribonuclease-sensitive ribosomal vaccine of Pseudomonas aeruginosa.铜绿假单胞菌的核糖核酸酶敏感核糖体疫苗。
Infect Immun. 1980 Jan;27(1):204-10. doi: 10.1128/iai.27.1.204-210.1980.
7
Protection of suckling mice from experimental cholera by maternal immunization: comparison of the efficacy of whole-cell, ribosomal-derived, and enterotoxin immunogens.通过母体免疫保护乳鼠免受实验性霍乱：全细胞、核糖体衍生和肠毒素免疫原的疗效比较。
Infect Immun. 1974 Jul;10(1):167-72. doi: 10.1128/iai.10.1.167-172.1974.
8
Immunity to Salmonella typhimurium infection: characterization of antigens in active protection by polyacrylamide gel electrophoresis.鼠伤寒沙门氏菌感染的免疫：通过聚丙烯酰胺凝胶电泳对主动保护中的抗原进行表征。
Infect Immun. 1973 Mar;7(3):507-11. doi: 10.1128/iai.7.3.507-511.1973.
9
Virulence factors involved in the intraperitoneal infection of adult mice with Vibrio cholerae.霍乱弧菌对成年小鼠腹腔感染所涉及的毒力因子。
Infect Immun. 1976 Feb;13(2):457-63. doi: 10.1128/iai.13.2.457-463.1976.
10
Evaluation of surface components of Vibrio cholerae as protective immunogens.霍乱弧菌表面成分作为保护性免疫原的评估。
Infect Immun. 1977 Feb;15(2):533-8. doi: 10.1128/iai.15.2.533-538.1977.
本文引用的文献
1
Protection against pneumococcal infection by a ribosomal preparation.通过核糖体制剂预防肺炎球菌感染。
Infect Immun. 1971 Jan;3(1):16-23. doi: 10.1128/iai.3.1.16-23.1971.
2
Immunogenic mycobacterial ribosomal and ribonucleic Acid preparations: chemical and physical characteristics.免疫原性分枝杆菌核糖体和核糖核酸制剂：化学和物理特性。
Infect Immun. 1970 Nov;2(5):659-68. doi: 10.1128/iai.2.5.659-668.1970.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS.从结核分枝杆菌获得的核糖体组分的免疫原活性
J Bacteriol. 1965 May;89(5):1291-8. doi: 10.1128/jb.89.5.1291-1298.1965.
5
A new modification of the carbazole analysis: application to heteropolysaccharides.咔唑分析的一种新改进：应用于杂多糖。
Anal Biochem. 1968 Sep;24(3):470-81. doi: 10.1016/0003-2697(68)90154-1.
6
7
Immunity induced by ribosomal extracts from Staphylococcus aureus.金黄色葡萄球菌核糖体提取物诱导的免疫
J Reticuloendothel Soc. 1970 Jul;8(1):66-73.
8
Dynamics of the 19S and 7S hemolysin responses in rabbits.
J Infect Dis. 1970 May;121(5):545-9. doi: 10.1093/infdis/121.5.545.
9
Antibacterial immunity induced by ribosomal vaccines.核糖体疫苗诱导的抗菌免疫
J Reticuloendothel Soc. 1970 Jul;8(1):13-24.
10
The antigens of Vibrio cholerae involved in the vibriocidal action of antibody and complement.霍乱弧菌的抗原参与抗体和补体的杀弧菌作用。
J Infect Dis. 1970 May;121(5):505-13. doi: 10.1093/infdis/121.5.505.
Zotero 插件