Meites S, Saniel-Banrey K
Clin Chem. 1979 Apr;25(4):531-4.
Of several preservatives we tested, a mixture of "isotonic" sodium fluoride and sodium iodoacetate (17.7 and 5.0 g/L, respectively) best inhibits glucose loss from blood collected from the newborn. However, the inhibition is incomplete. Twenty microliters of blood is diluted with 100 microliter of preserving solution. After centrifugation, the glucose in 100 microliter of prewarmed supernate (diluted plasma) is measured in a Beckman Glucose Analyzer, based on the action of a glucose reagent and the rate of oxygen consumption. The method, in effect, determines the glucose in whole blood, except for about 5% of the total that remains in the erythrocytes. Glucose is distributed in blood according to the water content of cells and plasma. From concentrations found in cells and plasma, we can calculate the value for glucose in whole blood with an accuracy of 95.2%. The modified method we describe shows acceptable precision, as judged from within-run and day-to-day CV--generally well below 5% for each.
在我们测试的几种防腐剂中，“等渗”氟化钠和碘乙酸钠的混合物（分别为17.7克/升和5.0克/升）能最好地抑制从新生儿采集的血液中的葡萄糖流失。然而，这种抑制并不完全。将20微升血液用100微升保存液稀释。离心后，基于葡萄糖试剂的作用和耗氧率，在贝克曼葡萄糖分析仪中测量100微升预热的上清液（稀释血浆）中的葡萄糖。实际上，该方法测定的是全血中的葡萄糖，但红细胞中仍残留约5%的葡萄糖总量。葡萄糖根据细胞和血浆的含水量在血液中分布。根据细胞和血浆中的浓度，我们可以计算出全血中葡萄糖的值，准确度为95.2%。从批内和日间变异系数判断，我们描述的改良方法显示出可接受的精密度——通常每种情况均远低于5%。
