Loganathan Preethi, Gasper Sampath Kumar, Afel Fathima Kader, Kandaswamy Selvakumar
1Department of Biochemistry, Bharathi Womens College, Chennai, India.
Department of Biochemistry, Vel's University, Chennai, India.
Indian J Clin Biochem. 2020 Jan;35(1):32-42. doi: 10.1007/s12291-018-0782-6. Epub 2018 Jul 21.
Status of diabetes of an individual is majorly evaluated by the frequent monitoring of glucose estimation. Use of serum samples and inappropriate plasma for estimating glucose is an existing practise in Indian standard of laboratories. There is a strong evidence for occurrence of in vitro glycolysis on the above mentioned specimens. The aim is to study the pre-analytical variations on the glucose estimation of using sodium fluoride-disodium EDTA (NaF-Na2EDTA) plasma (glycolysis inhibiting anticoagulant) and determine the fact behind the activity of glycolysis inhibition on the same. Healthy volunteers 20-35 years of both genders consisting of 40 members were selected for the study, and after getting the informed consent form, random blood samples were collected to study the errors of pre-analytical i.e., mixing of NaF-Na2EDTA tube by phlebotomist (no of inversion). Difference in duration from blood collection to centrifugation and a variable in time were taken from centrifugation to analyzing the plasma sample. Comparative studies on EDTA plasma and serum sample were also carried out. The usage of the evacuated blood collection system on NaF-Na2EDTA was shown to have the complete glycolysis inhibitor among all pre-analytical errors, whereas other tubes shown considerable increase in glycolysis. Recently the use of glycolysis inhibitor tubes are come into practise only in accredited or certified laboratories. Hence the authentication of glycolysis inhibition study is mandatory for the pre-analytical variations on the same.
个体的糖尿病状况主要通过频繁监测血糖估计值来评估。在印度实验室标准中，使用血清样本和不适当的血浆来估计血糖是一种现有的做法。有强有力的证据表明上述标本会发生体外糖酵解。目的是研究使用氟化钠 - 乙二胺四乙酸二钠（NaF - Na₂EDTA）血浆（糖酵解抑制抗凝剂）进行血糖估计时的分析前变异，并确定其糖酵解抑制活性背后的事实。选择了40名年龄在20 - 35岁的男女健康志愿者进行研究，在获得知情同意书后，采集随机血样以研究分析前误差，即采血人员对NaF - Na₂EDTA管的混合（颠倒次数）。记录从采血到离心的持续时间差异以及从离心到分析血浆样本的时间变量。还对乙二胺四乙酸（EDTA）血浆和血清样本进行了比较研究。在所有分析前误差中，使用真空采血系统采集的NaF - Na₂EDTA显示具有完全的糖酵解抑制剂作用，而其他试管显示糖酵解有相当大的增加。最近，糖酵解抑制管仅在经过认可或认证的实验室中开始使用。因此，对于同一分析前变异，糖酵解抑制研究的验证是必不可少的。
