Bacon T H, de Vere-Tyndall A, Tyrrell D A, Denman A M, Ansell B M
Clin Exp Immunol. 1983 Oct;54(1):23-30.
Eighteen patients with active systemic juvenile chronic arthritis (JCA) were studied for periods of up to 1 year to see whether any relationships existed between the interferon (IFN) response, the course of the underlying disease and intercurrent infections. The control group consisted of 23 children who were each seen on one occasion. IFN-alpha production by mononuclear cells (MNC) cultured in vitro was stimulated with Newcastle disease virus (NDV). Comparison of the mean IFN-alpha responses from all patients with control responses showed that MNC from the JCA group produced significantly more IFN-alpha. Furthermore, MNC obtained from JCA patients at times of systemic flare produced significantly higher titres of IFN-alpha than at times when the patients were clinically stable. IFN-alpha production by MNC from individual patients fluctuated considerably from occasion to occasion whereas IFN-gamma production by MNC induced with phytohaemagglutinin (PHA) remained more stable. There was no significant difference between patients and controls with respect to IFN-gamma responses and no relationship with clinical condition. Serum IFN was not detected either by sensitive bioassays for IFN-alpha and IFN-gamma or by an immunoradiometric assay for IFN-alpha.
对18例活动性全身型幼年慢性关节炎（JCA）患者进行了长达1年的研究，以观察干扰素（IFN）反应、基础疾病病程和并发感染之间是否存在任何关联。对照组由23名儿童组成，每人仅接受一次检查。用新城疫病毒（NDV）刺激体外培养的单核细胞（MNC）产生α干扰素。将所有患者的平均α干扰素反应与对照反应进行比较，结果显示JCA组的MNC产生的α干扰素明显更多。此外，在全身发作时从JCA患者获得的MNC产生的α干扰素滴度明显高于患者临床稳定时。个体患者MNC产生的α干扰素在不同时间波动很大，而用植物血凝素（PHA）诱导的MNC产生的γ干扰素则保持更稳定。患者和对照在γ干扰素反应方面没有显著差异，且与临床状况无关。通过针对α干扰素和γ干扰素的灵敏生物测定法或针对α干扰素的免疫放射测定法均未检测到血清干扰素。
