Weissenbach J, Zeevi M, Landau T, Revel M
Eur J Biochem. 1979 Jul;98(1):1-8. doi: 10.1111/j.1432-1033.1979.tb13153.x.
Messenger RNA was purified from human foreskin fibroblasts FS11, a high interferon-producer line, after induction with synthetic double-stranded RNA. The mRNA was translated in a cell-free protein-synthesis system from rabbit reticulocytes. The translation products, containing biologically active human interferon, were immunoprecipitated by a serum from rabbits immunized against partially purified interferon. Analysis of the immunoprecipitate by polyacrylamide gel electrophoresis in dodecylsulfate shows that the product of human fibroblast interferon mRNA is a 23000-Mr polypeptide. Methods are described for the synthesis and rapid identification of this polypeptide, which should be useful for structural analysis of interferon and isolation of its mRNA.
在用合成双链RNA诱导后，从高干扰素产生系人包皮成纤维细胞FS11中纯化信使RNA。该信使RNA在兔网织红细胞的无细胞蛋白质合成系统中进行翻译。含有生物活性人干扰素的翻译产物，被用针对部分纯化干扰素免疫的兔血清进行免疫沉淀。通过十二烷基硫酸盐聚丙烯酰胺凝胶电泳对免疫沉淀物的分析表明，人成纤维细胞干扰素信使RNA的产物是一种分子量为23000的多肽。文中描述了该多肽的合成及快速鉴定方法，这对于干扰素的结构分析及其信使RNA的分离应是有用的。
