Winston S H, Rustigian R
Infect Immun. 1979 Jun;24(3):967-70. doi: 10.1128/iai.24.3.967-970.1979.
The effect of low doses of actinomycin D on Vero cell nontransmissible measles infection produced after cocultivation with a HeLa subline with persistent defective infection by Edmonston measles virus was examined by pretreatment of Vero cells with the drug and treatment at 1, 4, and 7 days after Vero cell infection. Pretreatment enhanced focal formation of viral immunofluorescent Vero cell syncytia but did not induce synthesis of detectable amounts od cocultures suppressed syncytial formation, and treatment of 4- and 7-day-old cocultures had little, if any, effect on syncytial formation. Pretreatment also eliminated a transient resistance to homologous superinfection. A possible relation of these findings to the presence of a cell-associated viral inhibitor in the persistently infected HeLa cells is discussed.
通过用放线菌素D预处理Vero细胞以及在Vero细胞感染后的第1、4和7天进行处理，研究了低剂量放线菌素D对与携带埃德蒙斯顿麻疹病毒持续性缺陷感染的HeLa亚系共培养后产生的Vero细胞非传染性麻疹感染的影响。预处理增强了病毒免疫荧光Vero细胞合胞体的灶性形成，但未诱导可检测量的共培养物中合胞体形成的抑制，并且对4日龄和7日龄共培养物的处理对合胞体形成几乎没有影响。预处理还消除了对同源超感染的短暂抗性。讨论了这些发现与持续感染的HeLa细胞中细胞相关病毒抑制剂存在的可能关系。
