Valenzuela P, Gray P, Quiroga M, Zaldivar J, Goodman H M, Rutter W J
Nature. 1979 Aug 30;280(5725):815-9. doi: 10.1038/280815a0.
DNA extracted from hepatitis B virus Dane particles has been cloned in bacteria using a plasmid vector. A full-length clone has been examined by restriction endonuclease analysis, and the nucleotide sequence of an 892-base pair fragment from cloned hepatitis B viral DNA encoding the surface antigen gene is reported. The amino acid sequence deduced from the DNA indicates that the surface antigens is a protein consisting of 226 amino acids and with a molecular weight of 25,398. The portion of the gene coding for this protein apparently contains no intervening sequences.
从乙肝病毒Dane颗粒中提取的DNA已利用质粒载体在细菌中克隆。通过限制性内切酶分析对一个全长克隆进行了检测，并报道了来自克隆的乙肝病毒DNA的一个编码表面抗原基因的892碱基对片段的核苷酸序列。从该DNA推导的氨基酸序列表明，表面抗原是一种由226个氨基酸组成、分子量为25398的蛋白质。编码该蛋白质的基因部分显然不包含居间序列。
